The pathological and molecular but not clinical phenotypes are maintained after second passage of experimental atypical bovine spongiform encephalopathy in cattle

BMC Veterinary Research

Research Article

Timm Konold¹  Derek Clifford¹  Saira Cawthraw²  Marion M Simmons³  Laura J Phelan³  Michael J Stack⁴  Melanie J Chaplin⁴ 

[1] Animal Sciences Unit, Animal Health and Veterinary Laboratories Agency, KT15 3NB, New Haw, Addlestone, UK;Central Sequencing Unit, Animal Health and Veterinary Laboratories Agency, KT15 3NB, New Haw, Addlestone, UK;Pathology Department, Animal Health and Veterinary Laboratories Agency, KT15 3NB, New Haw, Addlestone, UK;Prion Unit, Virology Department, Animal Health and Veterinary Laboratories Agency, KT15 3NB, New Haw, Addlestone, UK;

关键词: Prion Protein;    Bovine Spongiform Encephalopathy;    Longe Survival Time;    Clinical Duration;    Bovine Spongiform Encephalopathy Case;   

DOI  :  10.1186/s12917-014-0243-2

 received in 2014-07-15, accepted in 2014-09-24,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundAtypical bovine spongiform encephalopathies (BSEs), classified as H-type and L-type BSE based on the Western immunoblot profiles, are naturally occurring diseases in cattle, which are phenotypically different to classical BSE. Transmission studies in cattle using the intracerebral route resulted in disease where the phenotypes were maintained irrespective of BSE type but clinically affected cattle with a shorter survival time displayed a nervous form whereas cattle with a longer survival time displayed a dull form. A second transmission study is reported here where four cattle were intracerebrally inoculated with brain tissue from experimentally infected cattle presenting with either the nervous or dull form of H- or L-type BSE to determine whether the phenotype is maintained.ResultsThe four inoculated cattle were culled at 16.5-19.5 months post inoculation after presenting with difficulty getting up, a positive scratch response (all) and dullness (three cattle), which was not observed in two non-inoculated control cattle, each housed with either group of inoculated cattle. Only the inoculated cattle had detectable prion protein in the brain based on immunohistochemical examination, and the Western immunoblot profile was consistent with the H-type or L-type BSE of the respective donor cattle.ConclusionsSecond passage of H-type and L-type BSE in cattle produced a TSE where the majority of cattle displayed the dull form regardless of clinical disease form of the donor cattle. The pathological and molecular phenotypes of H- and L-type BSE were maintained.

【 授权许可】

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© Crown copyright; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.