A 2cM genome-wide scan of European Holstein cattle affected by classical BSE

BMC Genetics

Research Article

Zhiquan Wang¹  Brenda M Murdoch¹  Paul Stothard¹  Stephen S Moore¹  Aparna Prasad¹  Stephanie McKay²  Matthew Settles³  William W Laegreid⁴  John L Williams⁵  Michael L Clawson⁶ 

[1] Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Canada;Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Canada;Divisions of Animal Sciences, University of Missouri, Columbia, Missouri, USA;Department of Animal Science, Washington State University, Pullman, Washington, USA;Department of Veterinary Pathobiology, University of Illinois, Urbana, Illinois, USA;Parco Tecnologico Padano, Via Einstein, Polo Universitario, 26900, Lodi, Italy;USDA, ARS, US Meat Animal Research Center, Clay Center, Nebraska, USA;

关键词: Prion Protein;    Bovine Spongiform Encephalopathy;    Single Nucleotide Polymorphism Marker;    PRNP Gene;    Amyloid Beta Protein Precursor;   

DOI  :  10.1186/1471-2156-11-20

 received in 2009-06-01, accepted in 2010-03-29,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundClassical bovine spongiform encephalopathy (BSE) is an acquired prion disease that is invariably fatal in cattle and has been implicated as a significant human health risk. Polymorphisms that alter the prion protein of sheep or humans have been associated with variations in transmissible spongiform encephalopathy susceptibility or resistance. In contrast, there is no strong evidence that non-synonymous mutations in the bovine prion gene (PRNP) are associated with classical BSE disease susceptibility. However, two bovine PRNP insertion/deletion polymorphisms, one within the promoter region and the other in intron 1, have been associated with susceptibility to classical BSE. These associations do not explain the full extent of BSE susceptibility, and loci outside of PRNP appear to be associated with disease incidence in some cattle populations. To test for associations with BSE susceptibility, we conducted a genome wide scan using a panel of 3,072 single nucleotide polymorphism (SNP) markers on 814 animals representing cases and control Holstein cattle from the United Kingdom BSE epidemic.ResultsTwo sets of BSE affected Holstein cattle were analyzed in this study, one set with known family relationships and the second set of paired cases with controls. The family set comprises half-sibling progeny from six sires. The progeny from four of these sires had previously been scanned with microsatellite markers. The results obtained from the current analysis of the family set yielded both some supporting and new results compared with those obtained in the earlier study. The results revealed 27 SNPs representing 18 chromosomes associated with incidence of BSE disease. These results confirm a region previously reported on chromosome 20, and identify additional regions on chromosomes 2, 14, 16, 21 and 28. This study did not identify a significant association near the PRNP in the family sample set. The only association found in the PRNP region was in the case-control sample set and this was not significant after multiple test correction. The genome scan of the case-control animals did not identify any associations that passed a stringent genome-wide significance threshold.ConclusionsSeveral regions of the genome are statistically associated with the incidence of classical BSE in European Holstein cattle. Further investigation of loci on chromosomes 2, 14, 16, 20, 21 and 28 will be required to uncover any biological significance underlying these marker associations.

【 授权许可】

Unknown   
© Murdoch et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.