HBV inhibits apoB production via the suppression of MTP expression

Lipids in Health and Disease

Research

Xinghui Liu¹  Guo-sheng Gao²  Cheng-liang Zhu³  Fu-Bing Wang⁴ 

[1] Department of Clinical Laboratory, Dongfeng Hospital, Hubei University of Medicine, 442008, Shiyan, PR, China;Department of Clinical Laboratory, Ningbo NO.2 hospital, 315010, Ningbo, PR, China;Department of Clinical Laboratory, Renmin Hospital of Wuhan University, 430060, Wuhan, Wuhan, PR, China;Department of Clinical Laboratory, Zhongnan Hospital, Wuhan University, 430071, Wuhan, PR, China;

关键词: hepatitisB virus;    chronic HBV infection;    lipid metabolism;    apolipoprotein B;    microsomal triglyceride transfer protein;   

DOI  :  10.1186/1476-511X-10-207

 received in 2011-10-05, accepted in 2011-11-11,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundLiver dominates the production and secretion of apolipoprotein B (apoB) and evidence shows that liver malfunction induced by hepatitis B virus (HBV) infection could lead to apolipoprotein metabolism disorders. The present study was undertaken to assess the effects of HBV on apoB expression.MethodsClinical examination: serum apoB levels in patients with chronic HBV infection and in healthy individuals were measured by immunoturbidimetry using biochemical analyzer Olympus 5400. Cell study: mRNA and protein expression levels of apoB in HepG2 and HepG2.2.15 cells were measured by RT-PCR and Western blot. Alternatively, HBV infectious clone pHBV1.3 or control plasmid pBlue-ks were tranfected into HepG2 cells, and mRNA and protein expression levels of apoB, as well as the microsomal triglyceride transfer protein (MTP) in tranfected HepG2 cells were also measured by RT-PCR and western blot.ResultsSerum apoB level was much lower in chronic HBV patients as compared to healthy individuals (P < 0.05). Expression of apoB mRNA and protein was lower in HepG2.2.15 cells than in HepG2 cells. Similarly, expression of apoB mRNA and protein was lower in pHBV1.3 transfected HepG2 cells than in pBlue-ks transfected HepG2 cells. Expression of MTP mRNA and protein in pHBV1.3 transfected HepG2 cells was reduced in a dose-dependent fashion.ConclusionHBV infection plays an inhibitory effect on apoB expression.

【 授权许可】

Unknown   
© Wang et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.