HBV inhibits apoB production via the suppression of MTP expression

Lipids in Health and Disease

Guo-sheng Gao³  Xinghui Liu¹  Cheng-liang Zhu⁴  Fu-Bing Wang² 

[1] Department of Clinical Laboratory, Dongfeng Hospital, Hubei University of Medicine, Shiyan, 442008, PR China;Department of Clinical Laboratory, Zhongnan Hospital, Wuhan University, Wuhan, 430071, PR China;Department of Clinical Laboratory, Ningbo NO.2 hospital, Ningbo, 315010, PR China;Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, Wuhan 430060, PR China

关键词: microsomal triglyceride transfer protein;    apolipoprotein B;    lipid metabolism;    chronic HBV infection;    hepatitisB virus;   

Others  :  1212430
DOI  :  10.1186/1476-511X-10-207

 received in 2011-10-05, accepted in 2011-11-11,  发布年份 2011

【 摘 要 】

Background

Liver dominates the production and secretion of apolipoprotein B (apoB) and evidence shows that liver malfunction induced by hepatitis B virus (HBV) infection could lead to apolipoprotein metabolism disorders. The present study was undertaken to assess the effects of HBV on apoB expression.

Methods

Clinical examination: serum apoB levels in patients with chronic HBV infection and in healthy individuals were measured by immunoturbidimetry using biochemical analyzer Olympus 5400. Cell study: mRNA and protein expression levels of apoB in HepG2 and HepG2.2.15 cells were measured by RT-PCR and Western blot. Alternatively, HBV infectious clone pHBV1.3 or control plasmid pBlue-ks were tranfected into HepG2 cells, and mRNA and protein expression levels of apoB, as well as the microsomal triglyceride transfer protein (MTP) in tranfected HepG2 cells were also measured by RT-PCR and western blot.

Results

Serum apoB level was much lower in chronic HBV patients as compared to healthy individuals (P < 0.05). Expression of apoB mRNA and protein was lower in HepG2.2.15 cells than in HepG2 cells. Similarly, expression of apoB mRNA and protein was lower in pHBV1.3 transfected HepG2 cells than in pBlue-ks transfected HepG2 cells. Expression of MTP mRNA and protein in pHBV1.3 transfected HepG2 cells was reduced in a dose-dependent fashion.

Conclusion

HBV infection plays an inhibitory effect on apoB expression.

【 授权许可】

   
2011 Wang et al; licensee BioMed Central Ltd.

【 图 表 】

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