Monocyte chemoattractant protein-1 is not required for liver regeneration after partial hepatectomy

Journal of Inflammation

Research

Cheri L. Lamb¹  Stephanie L. Wyler²  Shawna L. D’Ingillo²  Kristen A. Mitchell³ 

[1] Biomolecular Sciences Ph.D. Program, Boise State University, 83725, Boise, ID, USA;Department of Biological Sciences, Boise State University, 1910 University Drive, 83725-1515, Boise, ID, USA;Department of Biological Sciences, Boise State University, 1910 University Drive, 83725-1515, Boise, ID, USA;Biomolecular Sciences Ph.D. Program, Boise State University, 83725, Boise, ID, USA;

关键词: MCP-1;    CCR2;    Macrophages;    Kupffer cells;    Partial hepatectomy;    Liver regeneration;   

DOI  :  10.1186/s12950-016-0136-1

 received in 2015-12-30, accepted in 2016-08-16,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundLiver regeneration following 70 % partial hepatectomy (PH) requires the coordinated expression of soluble mediators produced by macrophages. Monocyte chemoattractant protein-1 (MCP-1) is a potent stimulus of monocyte recruitment and macrophage activation. The goal of this study was to determine how MCP-1 contributes to liver regeneration.MethodsPH was performed on anesthetized C57Bl/6 (wild type) and MCP-1 knockout mice, and macrophage-produced cytokines and hepatocyte proliferation were measured.ResultsIn wild type mice, hepatic MCP-1 protein levels increased 4–6 h after PH, and elevated plasma MCP-1 levels were detected 12 h after PH. Hepatocyte proliferation was comparable in MCP-1 knockout and wild type mice, as was the expression of macrophage-derived cytokines, TNFα and IL-6, and levels of phosphorylated STAT3. The number of CCR2+ cells in the liver was similar in MCP-1 knockout and wild type mice, which suggests that other chemokines may recruit CCR2+ cells in the absence of MCP-1. Studies with CCR2 knockout mice revealed that hepatocyte proliferation was suppressed ~40 % compared to wild type mice 36 h after PH, but proliferation and liver-body-weight ratios were similar at 48 h.ConclusionThese findings suggest that MCP-1 is not required for PH-induced liver regeneration, yet the role of CCR2 warrants further study.

【 授权许可】

CC BY   
© The Author(s). 2016