Spatio-temporal trends and risk factors affecting West Nile virus and related flavivirus exposure in Spanish wild ruminants

BMC Veterinary Research

Research Article

Steeve Lowenski¹  Sylvie Lecollinet¹  Jorge Paniagua²  Antonio Arenas-Montes²  David Cano-Terriza²  Ignacio García-Bocanegra²  Christian Gortázar³  Ursula Höfle³  Ana V. Gutiérrez-Guzmán³  Mariana Boadella⁴ 

[1] ANSES, Laboratoire de Santé Animale de Maisons-Alfort, UMR 1161 Virologie, INRA, ANSES, ENVA, F-94703, Maisons-Alfort, France;Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad de Córdoba-Agrifood Excellence International Campus (ceiA3), Rabanales, 14071, Córdoba, Spain;Instituto de Investigación en Recursos Cinegéticos IREC, (CSIC-UCLM-JCCM), Ciudad Real, Spain;Sabiotec, Camino de Moledores s.n., Ed. Polivalente UCLM, 13005, Ciudad Real, Spain;

关键词: West Nile virus;    Usutu virus;    Meaban virus;    Cervus elaphus;    Risk factors;    Wild ruminants;    Spain;   

DOI  :  10.1186/s12917-016-0876-4

 received in 2016-06-26, accepted in 2016-10-29,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundDuring the last decade, the spread of many flaviviruses (Genus Flavivirus) has been reported, representing an emerging threat for both animal and human health. To further study utility of wild ruminant samples in West Nile virus (WNV) surveillance, we assessed spatio–temporal trends and factors associated with WNV and cross-reacting flaviviruses exposure, particularly Usutu virus (USUV) and Meaban virus (MBV), in wild ruminants in Spain. Serum samples from 4693 wild ruminants, including 3073 free-living red deer (Cervus elaphus), 201 fallow deer (Dama dama), 125 mouflon (Ovis aries musimon), 32 roe deer (Capreolus capreolus) and 1262 farmed red deer collected in 2003–2014, were screened for WNV and antigenically-related flavivirus antibodies using a blocking ELISA (bELISA). Positive samples were tested for neutralizing antibodies against WNV, USUV and MBV by virus micro-neutralization tests.ResultsMean flavivirus seroprevalence according to bELISA was 3.4 ± 0.5 % in red deer, 1.0 ± 1.4 % in fallow deer, 2.4 ± 2.7 % in mouflon and 0 % in roe deer. A multivariate logistic regression model revealed as main risk factors for seropositivity in red deer; year (2011), the specific south-coastal bioregion (bioregion 5) and presence of wetlands. Red deer had neutralizing antibodies against WNV, USUV and MBV.ConclusionsThe results indicate endemic circulation of WNV, USUV and MBV in Spanish red deer, even in areas without known flavivirus outbreaks. WNV antibodies detected in a free-living red deer yearling sampled in 2010, confirmed circulation this year. Co-circulation of WNV and USUV was detected in bioregions 3 and 5, and of WNV and MBV in bioregion 3. Sampling of hunted and farmed wild ruminants, specifically of red deer yearlings, could be a complementary way to national surveillance programs to monitor the activity of emerging flaviviruses.

【 授权许可】

CC BY   
© The Author(s). 2016