Widespread Circulation of Flaviviruses in Horses and Birds in Northeastern Spain (Catalonia) between 2010 and 2019

Viruses

Elena Obón¹  Rafael Molina-López¹  Olga Nicolás²  Maria Pifarré³  Francisco Llorente⁴  Miguel Ángel Jiménez-Clavero⁴  Pilar Aguilera-Sepúlveda⁴  Eduard Jose-Cunilleras⁵  Lola Pailler-García⁶  Núria Busquets⁶  Sebastian Napp⁶  Mercè Soler⁷  Cécile Beck⁸  Rayane Amaral⁸  Sylvie Lecollinet⁸ 

[1] Centre de Fauna de Torreferrussa, Àrea de Gestió Ambiental Servei de Fauna i Flora, Forestal Catalana, 08130 Santa Perpètua de Mogoda, Spain;Centre de Fauna de Vallcalent, Àrea de Gestió Ambiental Servei de Fauna i Flora, Forestal Catalana, 25199 Lleida, Spain;Centre de Fauna dels Aiguamolls de l’Empordà, Àrea de Gestió Ambiental Servei de Fauna i Flora, Forestal Catalana, 17486 Castelló d’Empúries, Spain;Centro de Investigación en Sanidad Animal (CISA), Instituto de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC), 28130 Valdeolmos, Spain;Departament de Medicina i Cirurgia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain;IRTA, Animal Health Research Centre (CReSA IRTA-UAB), 08193 Bellaterra, Spain;Servei de Prevenció en Salut Animal, Departament d’Acció Climàtica, Alimentació i Agenda Rural (DACC), 08007 Barcelona, Spain;UMR 1161 Virology, ANSES, INRAE, ENVA, ANSES Animal Health Laboratory, EURL for Equine Diseases, 94704 Maisons-Alfort, France;

关键词: West Nile virus;    Usutu virus;    Bagaza virus;    tick-borne encephalitis virus;    flaviviruses;    Spain;   

DOI  :  10.3390/v13122404

来源: DOAJ

【 摘 要 】

The surveillance for West Nile virus (WNV) in Catalonia (northeastern Spain) has consistently detected flaviviruses not identified as WNV. With the aim of characterizing the flaviviruses circulating in Catalonia, serum samples from birds and horses collected between 2010 and 2019 and positive by panflavivirus competition ELISA (cELISA) were analyzed by microneutralization test (MNT) against different flaviviruses. A third of the samples tested were inconclusive by MNT, highlighting the limitations of current diagnostic techniques. Our results evidenced the widespread circulation of flaviviruses, in particular WNV, but also Usutu virus (USUV), and suggest that chicken and horses could serve as sentinels for both viruses. In several regions, WNV and USUV overlapped, but no significant geographical aggregation was observed. Bagaza virus (BAGV) was not detected in birds, while positivity to tick-borne encephalitis virus (TBEV) was sporadically detected in horses although no endemic foci were observed. So far, no human infections by WNV, USUV, or TBEV have been reported in Catalonia. However, these zoonotic flaviviruses need to be kept under surveillance, ideally within a One Health framework.

【 授权许可】

Unknown