Transcriptome analysis of paired primary colorectal carcinoma and liver metastases reveals fusion transcripts and similar gene expression profiles in primary carcinoma and liver metastases

BMC Cancer

Research Article

Nahmgun Oh¹  Hong-Jae Jo¹  Hyun Sung Kim¹  Do Youn Park²  Ja-Rang Lee²  Chae Hwa Kwon²  Yuri Choi²  Hye Ji Park² 

[1] BioMedical Research Institute Pusan National University Hospital, Busan, Seo-Gu, Korea;Department of Surgery Pusan National University Hospital, Pusan National University School of Medicine, Busan, Seo-Gu, Korea;Department of Pathology Pusan National University Hospital, Pusan National University School of Medicine, 602-739, Busan, Seo-Gu, Korea;BioMedical Research Institute Pusan National University Hospital, Busan, Seo-Gu, Korea;

关键词: Colorectal cancer;    RNA-seq;    Expression profiling;    Gene fusion;   

DOI  :  10.1186/s12885-016-2596-3

 received in 2015-09-09, accepted in 2016-07-21,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundDespite the clinical significance of liver metastases, the difference between molecular and cellular changes in primary colorectal cancers (CRC) and matched liver metastases is poorly understood.MethodsIn order to compare gene expression patterns and identify fusion genes in these two types of tumors, we performed high-throughput transcriptome sequencing of five sets of quadruple-matched tissues (primary CRC, liver metastases, normal colon, and liver).ResultsThe gene expression patterns in normal colon and liver were successfully distinguished from those in CRCs; however, RNA sequencing revealed that the gene expression between primary CRCs and their matched liver metastases is highly similar. We identified 1895 genes that were differentially expressed in the primary carcinoma and liver metastases, than that in the normal colon tissues. A major proportion of the transcripts, identified by gene expression profiling as significantly enriched in the primary carcinoma and metastases, belonged to gene ontology categories involved in the cell cycle, mitosis, and cell division. Furthermore, we identified gene fusion events in primary carcinoma and metastases, and the fusion transcripts were experimentally confirmed. Among these, a chimeric transcript resulting from the fusion of RNF43 and SUPT4H1 was found to occur frequently in primary colorectal carcinoma. In addition, knockdown of the expression of this RNF43-SUPT4H1 chimeric transcript was found to have a growth-inhibitory effect in colorectal cancer cells.ConclusionsThe present study reports a high concordance of gene expression in the primary carcinoma and liver metastases, and reveals potential new targets, such as fusion genes, against primary and metastatic colorectal carcinoma.

【 授权许可】

CC BY   
© The Author(s). 2016