CTX-M-14 β-lactamase-producing Citrobacter freundii isolated in Venezuela

Annals of Clinical Microbiology and Antimicrobials

Case Report

Bárbara Ghiglione¹  Gabriel Gutkind¹  María Araque²  Beatriz Millán²  Tulia Díaz³ 

[1] Cátedra de Microbiología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina;Laboratorio de Microbiología Molecular, Facultad de Farmacia y Bioanálisis, Universidad de Los Andes, Mérida, Venezuela;Laboratorio de Microbiología Molecular, Facultad de Farmacia y Bioanálisis, Universidad de Los Andes, Mérida, Venezuela;Laboratorio de Síndromes Gastrointestinales y Urinarios, Facultad de Farmacia y Bioanálisis, Universidad de Los Andes, Mérida, Venezuela;

关键词: Citrobacter freundii;    Extended-spectrum β-lactamases;    CTX-M-14, plasmid;    FrepB replicon;   

DOI  :  10.1186/1476-0711-10-22

 received in 2011-04-27, accepted in 2011-05-31,  发布年份 2011

来源: Springer

【 摘 要 】

A clinical isolate of C. freundii with reduced susceptibility to extended-spectrum β-lactams from a woman with cystocele associated with recurrent urinary tract infection was analyzed. Susceptibility tests, double disk synergy tests (DDST) and enzymatic activity by the agar iodometric method suggested the presence of ESBLs. Conjugation experiments revealed the presence of a large conjugative plasmid (pLM07/20) with an exclusive FrepB replicon type (IncF/FIB). PCR analysis and sequencing confirmed the presence of the blaCTX-M-14 gene in the pLM07/20 from C. freundii.LM07/10. Although this is the first report of CTX-M-14 in Venezuela, we alert the medical community that future increase of these β-lactamases in our city could be due to dissemination of plasmids into bacterial populations.

【 授权许可】

Unknown   
© Millán et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.