Whole-genome discovery of miRNAs and their targets in wheat (Triticum aestivum L.)

BMC Plant Biology

Research Article

Weiwei Guo¹  Yingyin Yao¹  Guanghui Guo¹  Qixin Sun¹  Huiru Peng¹  Zhongfu Ni¹  Fenglong Sun¹  Jinkun Du¹ 

[1] State Key Laboratory for Agrobiotechnology and Key Laboratory of Crop Heterosis and Utilization (MOE) and Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, 100193, Beijing, PR China;

关键词: miRNA Family;    Wheat Genome;    Conserve miRNA Family;    Degradome Sequencing;    Degradome Library;   

DOI  :  10.1186/1471-2229-14-142

 received in 2014-01-25, accepted in 2014-05-19,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundMicroRNAs (miRNAs) are small, non-coding RNAs playing essential roles in plant growth, development, and stress responses. Sequencing of small RNAs is a starting point for understanding their number, diversity, expression and possible roles in plants.ResultsIn this study, we conducted a genome-wide survey of wheat miRNAs from 11 tissues, characterizing a total of 323 novel miRNAs belonging to 276 families in wheat. A miRNA conservation analysis identified 191 wheat-specific miRNAs, 2 monocot-specific miRNAs, and 30 wheat-specific variants from 9 highly conserved miRNA families. To understand possible roles of wheat miRNAs, we determined 524 potential targets for 124 miRNA families through degradome sequencing, and cleavage of a subset of them was validated via 5′ RACE. Based on the genome-wide identification and characterization of miRNAs and their associated target genes, we further identified 64 miRNAs preferentially expressing in developing or germinating grains, which could play important roles in grain development.ConclusionWe discovered 323 wheat novel miRNAs and 524 target genes for 124 miRNA families in a genome-wide level, and our data will serve as a foundation for future research into the functional roles of miRNAs in wheat.

【 授权许可】

CC BY   
© Sun et al.; licensee BioMed Central Ltd. 2014