Identification of wounding and topping responsive small RNAs in tobacco (Nicotiana tabacum)

BMC Plant Biology

Research Article

Qian-Hao Zhu¹  Yijie Gui²  Zefeng Li²  Longjiang Fan²  Yu Wang²  She Tang²  Jiahua Xie³  Bingguang Xiao⁴ 

[1] CSIRO Plant Industry, Canberra ACT 2601, Australia;Department of Agronomy and James D. Watson Institute of Genome Sciences, Zhejiang University, 310058, Hangzhou, Zhejiang, China;Department of Pharmaceutical Sciences, North Carolina Central University, 27707, Durham, NC, USA;Yunnan Academy of Tobacco Agricultural Sciences and China Tobacco Breeding Research Center at Yunnan, 653100, Yuxi, Yunnan, China;

关键词: Small RNAs;    miRNA Family;    Damage Treatment;    Conserve miRNA Family;    Degradome Sequencing;   

DOI  :  10.1186/1471-2229-12-28

 received in 2011-06-12, accepted in 2012-02-22,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundMicroRNAs (miRNAs) and short interfering RNAs (siRNAs) are two major classes of small RNAs. They play important regulatory roles in plants and animals by regulating transcription, stability and/or translation of target genes in a sequence-complementary dependent manner. Over 4,000 miRNAs and several classes of siRNAs have been identified in plants, but in tobacco only computational prediction has been performed and no tobacco-specific miRNA has been experimentally identified. Wounding is believed to induce defensive response in tobacco, but the mechanism responsible for this response is yet to be uncovered.ResultsTo get insight into the role of small RNAs in damage-induced responses, we sequenced and analysed small RNA populations in roots and leaves from wounding or topping treated tobacco plants. In addition to confirmation of expression of 27 known miRNA families, we identified 59 novel tobacco-specific miRNA members of 38 families and a large number of loci generating phased 21- or 24-nt small RNAs (including ta-siRNAs). A number of miRNAs and phased small RNAs were found to be responsive to wounding or topping treatment. Targets of small RNAs were further surveyed by degradome sequencing.ConclusionsThe expression changes of miRNAs and phased small RNAs responsive to wounding or topping and identification of defense related targets for these small RNAs suggest that the inducible defense response in tobacco might be controlled by pathways involving small RNAs.

【 授权许可】

Unknown   
© Tang et al; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.