Jasmonate-mediated defence responses, unlike salicylate-mediated responses, are involved in the recovery of grapevine from bois noir disease

BMC Plant Biology

Research Article

Maurizio Badiani¹  Stefania Pasqualini²  Luisa Ederli²  Simonetta Santi³  Rita Musetti³  Claudia Zadra⁴  Anna Rita Paolacci⁵  Giulio Catarcione⁵  Mario Ciaffi⁵ 

[1] Dipartimento di Agraria, Università Mediterranea di Reggio Calabria, Loc. Feo di Vito, I-89129, Reggio Calabria, Italy;Dipartimento di Chimica, Biologia e Biotecnologie, Università di Perugia, Borgo XX Giugno, 74, I-06121, Perugia, Italy;Dipartimento di Scienze Agroalimentari, Ambientali e Animali, Università di Udine, Via delle Scienze, 206, I-33100, Udine, Italy;Dipartimento di Scienze Farmaceutiche, Università di Perugia, Borgo XX Giugno, 74, I-06121, Perugia, Italy;Dipartimento per la Innovazione nei Sistemi Biologici, Agroalimentari e Forestali, Università della Tuscia, Via S. Camillo De Lellis, s.n.c, I-01100, Viterbo, Italy;

关键词: Bois noir;    Grapevine;    Jasmonate;    Phytoplasmas;    Plant-pathogen interactions;    Salicylate;    Stolbur;    Vitis vinifera;   

DOI  :  10.1186/s12870-017-1069-4

 received in 2017-03-14, accepted in 2017-06-25,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundBois noir is an important disease of grapevine (Vitis vinifera L.), caused by phytoplasmas. An interesting, yet elusive aspect of the bois noir disease is “recovery”, i.e., the spontaneous and unpredictable remission of symptoms and damage. Because conventional pest management is ineffective against bois noir, deciphering the molecular bases of recovery is beneficial. The present study aimed to understand whether salicylate- and jasmonate-defence pathways might have a role in the recovery from the bois noir disease of grapevine.ResultsLeaves from healthy, bois noir-diseased and bois noir-recovered plants were compared, both in the presence (late summer) and absence (late spring) of bois noir symptoms on the diseased plants. Analyses of salicylate and jasmonate contents, as well as the expression of genes involved in their biosynthesis, signalling and action, were evaluated. In symptomatic diseased plants (late summer), unlike symptomless plants (late spring), salicylate biosynthesis was increased and salicylate-responsive genes were activated. In contrast, jasmonate biosynthesis and signalling genes were up-regulated both in recovered and diseased plants at all sampling dates. The activation of salicylate signalling in symptomatic plants might have antagonised the jasmonate-mediated defence response by suppressing the expression of jasmonate-responsive genes.ConclusionsOur results suggest that grapevine reacts to phytoplasma infection through salicylate-mediated signalling, although the resultant full activation of a salicylate-mediated response is apparently ineffective in conferring resistance against bois noir disease. Activation of the salicylate signalling pathway that is associated with the presence of bois noir phytoplasma seems to antagonise the jasmonate defence response, by failing to activate or suppressing both the expression of some jasmonate responsive genes that act downstream of the jasmonate biosynthetic pathway, as well as the first events of the jasmonate signalling pathway. On the other hand, activation of the entire jasmonate signalling pathway in recovered plants suggests the potential importance of jasmonate-regulated defences in preventing bois noir phytoplasma infections and the subsequent development of bois noir disease. Thus, on one hand, recovery could be achieved and maintained over time by preventing the activation of defence genes associated with salicylate signalling, and on the other hand, by activating jasmonate signalling and other defence responses.

【 授权许可】

CC BY   
© The Author(s). 2017