Geographical and Temporal Diversity of ‘Candidatus Phytoplasma solani' in Wine-Growing Regions in Slovenia and Austria

Frontiers in Plant Science

Aleš Kladnik¹  Günter Brader²  Marina Dermastia³  Sara Mermal⁴  Sara Vidmar⁴  Sanda Kavčič⁴  Maruša Pompe Novak⁵  Nataša Mehle⁵  Monika Riedle-Bauer⁶ 

[1] Bioresources, Austrian Institute of Technology, Tulln, Austria;;Center for Health &Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia;Department of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia;Faculty of Viticulture and Enology, University of Nova Gorica, Vipava, Slovenia;Federal College and Research Institute for Viticulture and Pomology Klosterneuburg, Klosterneuburg, Austria;

关键词: Bois noir;    genotyping;    ‘Ca. P. solani';    tuf gene;    secY;    survey;   

DOI  :  10.3389/fpls.2022.889675

来源: DOAJ

【 摘 要 】

As the causal agent of the grapevine yellows disease Bois noir, ‘Candidatus Phytoplasma solani' has a major economic impact on grapevines. To improve the control of Bois noir, it is critical to understand the very complex epidemiological cycles that involve the multiple “Ca. P. solani” host plants and insect vectors, of which Hyalesthes obsoletus is the most important. In the present study, multiple genotyping of the tuf, secY, stamp, and vmp1 genes was performed. This involved archived grapevine samples that were collected during an official survey of grapevine yellows throughout the wine-growing regions of Slovenia (from 2003 to 2016), plus samples from Austrian grapevines, stinging nettle, field bindweed, and insect samples (collected from 2012 to 2019). The data show that the tuf-b2 type of the tuf gene has been present in eastern Slovenia since at least 2003. The hypotheses that the occurrence of the haplotypes varies due to the geographical position of Slovenia on the Italian–Slovenian Karst divide and that the haplotypes are similar between Slovenian and Austrian Styria were confirmed. The data also show haplotype changes for host plants and H. obsoletus associated with ‘Ca. P. solani,' which might be linked to new epidemiological cycles of this phytoplasma that involve not just new plant sources and new insect vectors, but also climate and land-use changes.

【 授权许可】

Unknown