The transcription factor Maf-S regulates metabolic resistance to insecticides in the malaria vector Anopheles gambiae

BMC Genomics

Research Article

Jonathan D. Moore¹  Hilary Ranson²  Patricia Pignatelli²  Victoria A. Ingham²  Simon Wagstaff² 

[1] Earlham Institute, Norwich Research Park Innovation Centre, Colney Lane, NR4 7UH, Norwich, England;Liverpool School of Tropical Medicine, Pembroke Place, L35QA, Liverpool, England;

关键词: Insecticide resistance;    Transcriptional control;    Cross resistance;    Metabolic resistance;    Mosquito;    Anopheles gambiae;   

DOI  :  10.1186/s12864-017-4086-7

 received in 2017-05-15, accepted in 2017-08-24,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundMalaria control in Africa is dependent upon the use insecticides but intensive use of a limited number of chemicals has led to resistance in mosquito populations. Increased production of enzymes that detoxify insecticides is one of the most potent resistance mechanisms. Several metabolic enzymes have been implicated in insecticide resistance but the processes controlling their expression have remained largely elusive.ResultsHere, we show that the transcription factor Maf-S regulates expression of multiple detoxification genes, including the key insecticide metabolisers CYP6M2 and GSTD1 in the African malaria vector Anopheles gambiae. Attenuation of this transcription factor through RNAi induced knockdown reduced transcript levels of these effectors and significantly increased mortality after exposure to the pyrethroid insecticides and DDT (permethrin: 9.2% to 19.2% (p = 0.015), deltamethrin: 3.9% to 21.6% (p = 0.036) and DDT: 1% to 11.7% (p = <0.01), whilst dramatically decreasing mortality induced by the organophosphate malathion (79.6% to 8.0% (p = <0.01)). Additional genes regulated by Maf-S were also identified providing new insight into the role of this transcription factor in insects.ConclusionMaf-S is a key regulator of detoxification genes in Anopheles mosquitoes. Disrupting this transcription factor has opposing effects on the mosquito’s response to different insecticide classes providing a mechanistic explanation to the negative cross resistance that has been reported between pyrethroids and organophosphates.

【 授权许可】

CC BY   
© The Author(s). 2017