Prenatal caloric restriction alters lipid metabolism but not hepatic Fasn gene expression and methylation profiles in rats

BMC Genetics

Research Article

Joanna Nowacka-Woszuk¹  Zofia E. Madeja¹  Agata Chmurzynska² 

[1] Department of Genetics and Animal Breeding, Poznań University of Life Sciences, Wolynska 33, 60-637, Poznan, Poland;Department of Human Nutrition and Hygiene, Poznań University of Life Sciences, Wojska Polskiego 31, 60-624, Poznan, Poland;

关键词: Nutrigenomics;    Calorie-restricted diet;    Rat;    Liver;    Fasn;    Gene expression;    DNA methylation;    Lipid profile;   

DOI  :  10.1186/s12863-017-0544-0

 received in 2017-03-31, accepted in 2017-08-08,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundUndernutrition is an increasingly common problem. Insufficient calorie intake and nutrient deficiencies during pregnancy may have an impact not only on the mother, but may also alter metabolism in the infant. In this study, we have applied a calorie-restricted diet during gestation and examined its effect on hepatic Fasn mRNA and DNA methylation profiles in rats and their female progeny. The body composition and blood lipid profiles were also evaluated in both generations.ResultsThe results showed that the investigated diet regimen exerted a greater effect on the dams than on the offspring. We found that, in the calorie-restricted group, the transcript level of the Fasn gene in the liver increased in the mothers, while in the progeny it was only slightly enhanced. The implemented diet altered lipid profile in the dams by decreasing total cholesterol, HDL, and TG levels. An increase in LDL was noted in the offspring. No change in DNA methylation profile was observed in response to the calorie-restricted diet.ConclusionsCalorie restriction during pregnancy modified the hepatic Fasn mRNA transcript level and altered the blood cholesterol concentrations in dams, but there were no such effects in their four-week-old offspring. The examined dietary regimen had no effect on DNA methylation of the Fasn 5′-flanking region in the rat liver.

【 授权许可】

CC BY   
© The Author(s). 2017