Nongenotoxic effects and a reduction of the DXR-induced genotoxic effects of Helianthus annuus Linné (sunflower) seeds revealed by micronucleus assays in mouse bone marrow

BMC Complementary and Alternative Medicine

Research Article

Carlos Tadeu dos Santos Dias¹  Marielly Reis Resende²  Maria Cristina Costa Resck³  Luiz Silva Souza⁴  Thaísla Andrielle da Silva⁴  João Evangelista Fiorini⁴  Marcelo Fabiano Gomes Boriollo⁵  Nelma de Mello Silva Oliveira⁶ 

[1] Departamento de Ciências Exatas, Escola de Agricultura “Luiz de Queiroz”, Universidade de São Paulo (ESALQ/USP), Piracicaba, SP, Brazil;Laboratório de Ecotoxicologia e Microbiologia Ambiental, Faculdade de Tecnologia, Universidade Estadual de Campinas (UNICAMP), Limeira, São Paulo, Brazil;Laboratório de Farmacogenômica e Biologia Molecular, Faculdade de Ciências Médicas & Centro de Pesquisa e Pós–graduação, Universidade José do Rosário Vellano (UNIFENAS), Campus Universitário, Rod. MG 179, Km 0, CEP: 37130-000, Alfenas, MG, Brazil;Centro de Cirurgia Experimental e Farmacologia, Universidade Estadual de Campinas, Campinas (UNICAMP), São Paulo, Brazil;Laboratório de Farmacogenômica e Biologia Molecular, Faculdade de Ciências Médicas & Centro de Pesquisa e Pós–graduação, Universidade José do Rosário Vellano (UNIFENAS), Campus Universitário, Rod. MG 179, Km 0, CEP: 37130-000, Alfenas, MG, Brazil;Centro de Pesquisa e Pós–graduação em Ciência Animal, Área de Patologia e Farmacologia Animal, Universidade José do Rosário Vellano (UNIFENAS), Alfenas, Minas Gerais, Brazil;Laboratório de Farmacogenômica e Biologia Molecular, Faculdade de Ciências Médicas & Centro de Pesquisa e Pós–graduação, Universidade José do Rosário Vellano (UNIFENAS), Campus Universitário, Rod. MG 179, Km 0, CEP: 37130-000, Alfenas, MG, Brazil;Centro de Pesquisa e Pós–graduação em Ciência Animal, Área de Patologia e Farmacologia Animal, Universidade José do Rosário Vellano (UNIFENAS), Alfenas, Minas Gerais, Brazil;Centro de Cirurgia Experimental e Farmacologia, Universidade Estadual de Campinas, Campinas (UNICAMP), São Paulo, Brazil;Laboratório de Farmacogenômica e Biologia Molecular, Faculdade de Ciências Médicas & Centro de Pesquisa e Pós–graduação, Universidade José do Rosário Vellano (UNIFENAS), Campus Universitário, Rod. MG 179, Km 0, CEP: 37130-000, Alfenas, MG, Brazil;Laboratório de Ecotoxicologia e Microbiologia Ambiental, Faculdade de Tecnologia, Universidade Estadual de Campinas (UNICAMP), Limeira, São Paulo, Brazil;

关键词: Bone marrow;    Helianthus annuus;    Micronucleus assay;    Rodents;    Tincture;    Oil;   

DOI  :  10.1186/1472-6882-14-121

 received in 2013-08-22, accepted in 2014-03-27,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundThis research evaluated the genotoxicity of oil and tincture of H. annuus L. seeds using the micronucleus assay in bone marrow of mice. The interaction between these preparations and the genotoxic effects of doxorubicin (DXR) was also analysed (antigenotoxicity test).MethodsExperimental groups were evaluated at 24-48 h post treatment with N-Nitroso-N-ethylurea (positive control – NEU), DXR (chemotherapeutic), NaCl (negative control), a sunflower tincture (THALS) and two sources of sunflower oils (POHALS and FOHALS). Antigenotoxic assays were carried out using the sunflower tincture and oils separately and in combination with NUE or DXR.ResultsFor THALS, analysis of the MNPCEs showed no significant differences between treatment doses (250–2,000 mg.Kg-1) and NaCl. A significant reduction in MNPCE was observed when THALS (2,000 mg.Kg-1) was administered in combination with DXR (5 mg.Kg-1). For POHALS or FOHALS, analysis of the MNPCEs also showed no significant differences between treatment doses (250–2,000 mg.Kg-1) and NaCl. However, the combination DXR + POHALS (2,000 mg.Kg-1) or DXR + FOHALS (2,000 mg.Kg-1) not contributed to the MNPCEs reduction.ConclusionsThis research suggests absence of genotoxicity of THALS, dose-, time- and sex-independent, and its combination with DXR can reduce the genotoxic effects of DXR. POHALS and FOHALS also showed absence of genotoxicity, but their association with DXR showed no antigenotoxic effects.

【 授权许可】

CC BY   
© Boriollo et al.; licensee BioMed Central Ltd. 2014