Antimicrobial activities of endophytic fungi isolated from Ophiopogon japonicus (Liliaceae)

BMC Complementary and Alternative Medicine

Research Article

Dawei Zhang¹  Shunxing Guo¹  Juan Chen¹  Yongmei Xing¹  Chunlan Wang¹  Hanqiao Liang¹ 

[1] The Key Laboratory of Bioactive Substances and Resource Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, 100193, Beijing, People's Republic of China;

关键词: Ophiopogon japonicus;    Endophytic fungi;    Peptide deformylase;    Antimicrobial activity;   

DOI  :  10.1186/1472-6882-12-238

 received in 2012-05-17, accepted in 2012-11-08,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundDrug resistance in bacteria has become a global concern and the search for new antibacterial agents is urgent and ongoing. Endophytes provide an abundant reservoir of bioactive metabolites for medicinal exploitation, and an increasing number of novel compounds are being isolated from endophytic fungi. Ophiopogon japonicus, containing compounds with antibacterial activity, is a traditional Chinese medicinal plant used for eliminating phlegm, relieving coughs, latent heat in the lungs, and alleviating diabetes mellitus. We investigated the antimicrobial activities of 30 strains of O. japonicus.MethodsFungal endophytes were isolated from roots and stems of O. japonicus collected from Chongqing City, southwestern China. Mycelial extracts (MC) and fermentation broth (FB) were tested for antimicrobial activity using peptide deformylase (PDF) inhibition fluorescence assays and MTT cell proliferation assays.ResultsA total of 30 endophytic strains were isolated from O. japonicus; 22 from roots and eight from stems. 53.33% of the mycelial extracts (MC) and 33.33% of the fermentation broths (FB) displayed potent inhibition of PDF. 80% of MC and 33.33% of FB significantly inhibited Staphylococcus aureus. 70% of MC and 36.67% of FB showed strong activities against Cryptococcus neoformans. None showed influence on Escherichia coli.ConclusionThe secondary metabolites of endophytic fungi from O. japonicus are potential antimicrobial agents.

【 授权许可】

Unknown   
© Liang et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.