Antimicrobial activities of endophytic fungi isolated from Ophiopogon japonicus (Liliaceae)

BMC Complementary and Alternative Medicine

Chunlan Wang¹  Shunxing Guo¹  Dawei Zhang¹  Juan Chen¹  Yongmei Xing¹  Hanqiao Liang¹ 

[1] The Key Laboratory of Bioactive Substances and Resource Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100193, Peoples Republic of China

关键词: Antimicrobial activity;    Peptide deformylase;    Endophytic fungi;    Ophiopogon japonicus;   

Others  :  1231456
DOI  :  10.1186/1472-6882-12-238

 received in 2012-05-17, accepted in 2012-11-08,  发布年份 2012

【 摘 要 】

Background

Drug resistance in bacteria has become a global concern and the search for new antibacterial agents is urgent and ongoing. Endophytes provide an abundant reservoir of bioactive metabolites for medicinal exploitation, and an increasing number of novel compounds are being isolated from endophytic fungi. Ophiopogon japonicus, containing compounds with antibacterial activity, is a traditional Chinese medicinal plant used for eliminating phlegm, relieving coughs, latent heat in the lungs, and alleviating diabetes mellitus. We investigated the antimicrobial activities of 30 strains of O. japonicus.

Methods

Fungal endophytes were isolated from roots and stems of O. japonicus collected from Chongqing City, southwestern China. Mycelial extracts (MC) and fermentation broth (FB) were tested for antimicrobial activity using peptide deformylase (PDF) inhibition fluorescence assays and MTT cell proliferation assays.

Results

A total of 30 endophytic strains were isolated from O. japonicus; 22 from roots and eight from stems. 53.33% of the mycelial extracts (MC) and 33.33% of the fermentation broths (FB) displayed potent inhibition of PDF. 80% of MC and 33.33% of FB significantly inhibited Staphylococcus aureus. 70% of MC and 36.67% of FB showed strong activities against Cryptococcus neoformans. None showed influence on Escherichia coli.

Conclusion

The secondary metabolites of endophytic fungi from O. japonicus are potential antimicrobial agents.

【 授权许可】

   
2012 Liang et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

【 参考文献 】

• [1]Strobel G, Daisy B: Bioprospecting for microbial endophytes and their natural products. Microbiol Mol Biol Rev 2003, 67:491-502.
• [2]Ni ZW, Li GH, Zhao PJ, Shen YM: Antimicrobial components of the endophytic fungal strain chaetomium globosum Ly50′ from maytenus hookeri. Nat Prod Rev (in Chinese) 2008, 20:33-36.
• [3]Kim S, Shin DS, Lee T, Oh KB: Periconicins, two new fusicoccane diterpenes produced by an endophytic fungus Periconia sp. with antibacterial activity. J Nat Prod 2004, 67:448-450.
• [4]Vaz AB, Mota RC, Bomfim MR, Vieira ML, Zani CL, Rosa CA, Rosa LH: Antimicrobial activity of endophytic fungi associated with Orchidaceae in Brazil. Can J Microbiol 2009, 55:1381-1391.
• [5]Flora of China Editorial Committee of Chinese Academy of Sciences: Flora of China. Beijing: Science Press; 1978:130-164.
• [6]Adinolfi M, Parilli Y, Zhu YX: Terpenoid glycosides from Ophiopogon japonicus roots. Phytochemistry 1990, 29:1696-1699.
• [7]Zhu Y, Yan K, Tu G: Two homoisoflavones from Ophiopogon japonicus. Phytochemistry 1987, 26:2873-2874.
• [8]Chang HM, But PP: Pharmacology and applications of Chinese materia medica. Singapore: World Scientific Publishing; 1986:543.
• [9]Xie W, Zhao Y, Zhang Y: Traditional Chinese medicines in treatment of patients with type 2 diabetes mellitus 2011. Evidence-Based Complementary and Alternative Medicine 2011, Article ID 726723:13.
• [10]Rao Y, Chen C, Zhao B: Study on the eliminating phlegm effect and bacteriostatic action of compound Maidong pills. Chin Hosp Pharm J 2008, 28(8):615-617.
• [11]Zhang YM, Li RH, Yang ZL: Study on the bacteriostasis of some kinds of Chinese herbal medicine extracting solution. J Anhui Agri Sci 2007, 35:10594-10595.
• [12]Lin D: Study on allelopathy of dwarf lilyturf (Ophiopogon japonicus Ker-Gawl.). In MS thesis. Japan: Miyazaki University; 2001:15-47.
• [13]Lin D, Tsuzuki E, Sugimoto Y, Matsuo M: Effects of methanol extracts from Ophiopogon japonicus on rice blast fungus. International Rice Research Notes 2003, 28:27-28.
• [14]Lin D, Dong Y, Xu R: Preliminary study on the antifungal activity of the extracts from dwarf lilyturf (ophiopogon japonicus K.) roots on three kinds of plant pathogen. J Anhui Agri Sci 2009, 37(9):4187-4188.
• [15]Nakanishi Y, Kaneda H: The chemical constitution research of Ophiopogon (Chinese). Yakugaku Zasshi 1987, 107:780-784.
• [16]Kou J, Sun Y, Lin Y, Cheng Z, Zheng W, Yu B, Xu Q: Anti-inflammatory activities of aqueous extract from Radix Ophiopogon japonicus and its two constituents. Biol Pharm Bull 2005, 28:1234-1238.
• [17]Hung TM, Cao VT, Nguyen TD, Ryoo SW, Lee JH, Kim JC, Minkyun N, Jung HJ, KiHwan B, Byung SM: Homoisoflavonoid derivatives from the roots of Ophiopogon japonicus and their in vitro anti-inflammation activity. BMC Letters 2010, 20:2412-2416.
• [18]Xu LL, Han L, Wu JZ, Zhang QY, Zhang H, Huang BK, Rahman K, Qin LP: Comparative research of chemical constituents, antifungal and antitumor properties of ether extracts of Panx ginseng and its endophytic fungus. Phytomedicine 2009, 16:609-616.
• [19]Apfel C, Banner DW, Bur D, Dietz M, Hirata T, Hubschwerlen C, Locher H, Page MGP, Pirson W, Rosse G, Specklin JL: Hydroxamic acid derivatives as potent peptide deformylase inhibitors and antibacterial agents. J Med Chem 2000, 43:2324-2331.
• [20]Arnold AE, Maynard Z, Gilbert GS, Coley PD, Kursat TA: Are tropical fungal endophytes hyperdiverse. Ecol Lett 2000, 3:267-274.
• [21]Bills GF: Isolation and analysis of endophytic fungal communities from woody plants. In Endophytic fungi in grasses and woody plants: Systematics ecology and evolution. Edited by Redlin SC, Carris LM. St. Paul, MN: APS Press; 1996:31-65.
• [22]Chen J, Hu KX, Hou XQ, Guo SX: Endophytic fungi assemblages from 10 Dendrobium medicinal plants (Orchidaceae). World J Microbiol Biotechnol 2011, 27:1009-1016.
• [23]Wang FW, Jiao RH, Cheng AB, Tan SH, Song YC: Antimicrobial potentials of endophytic fungi residing in Quercus variabilis and brefeldin A obtained from Cladosporium sp. World J Microbiol Biotechnol 2006, 235:79-83.
• [24]Giglione C, Pierre M, Meinnel T: Peptide deformylase as a target for new generation, broad spectrum antimicrobial agents. Mol Microbiol 2000, 36:1197-1205.
• [25]Pei D: Peptide deformylase: A target for novel antibiotics. Emerging Ther Targets 2001, 5:23-40.
• [26]Yuan Z, Trias J, White RJ: Deformylase as a novel antibacterial target. Drug Discov Today 2001, 6:954-961.
• [27]Clements JM, Beckett RP, Brown A, Catlin G, Lobell M, Palan S, Thomas W, Whittaker M, Wood S, Salama S, Baker PJ, Rodgers HF, Barynin V, Rice DW, Hunter MG: Antibiotic activity and characterization of BB-3497, a novel peptide deformylase inhibitor. Antimicrob Agents Chemother 2001, 45:563-570.
• [28]Ki HN, Jung H, Amit P, Eunice EK, NamHyun C, Kwang YH: Insight into the antibacterial drug design and architectural mechanism of peptide recognition from the E. faecium peptide deformylase structure. Proteins 2009, 74:261-265.
• [29]Xian BT, Shu YS, Yue QZ: Identification of a New peptide deformylase gene from Enterococcus faecium and establishment of a New screening model targeted on PDF for novel antibiotics. Biomed Environ Sci 2004, 17:350-358.
• [30]Alicia FSM, Patricia O, Jon V: Over-expression of peptide deformylase in chloroplasts confers actinonin resistance, but is not a suitable selective marker system for plastid transformation. Transgenic Res 2011, 20:613-624.
• [31]Mosmann T: Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983, 65:55-63.
• [32]Denizot F, Lang R: Rapid colorimetric assay for cell growth and survival. J Immunol Methods 1986, 89:271-277.
• [33]Wang FY, Cao LT, Hu SH: A rapid and accurate 3-(4, 5-dimethyl thiazol-2-yl)-2, 5-diphenyl tetrazolium bromide colorimetric assay for quantification of bacteriocins with nisin as an example. J Zhejiang Univ Sci B 2007, 8:549-554.
• [34]Zgoda JR, Porter JR: A Convenient microdilution method for screening natural products against bacteria and fungi. Pharm Biol 2001, 39:221-225.
• [35]Xing YM, Chen J, Cui JL, Chen XM, Guo SX: Antimicrobial activity and biodiversity of endophytic fungi in Dendrobium devonianum and Dendrobium thyrsiflorum from Vietnam. Curr Microbiol 2011, 62:1218-1224.
• [36]Cui JL, Guo SX, Dong HL, Xiao PG: Endophytic fungi from Dragon’s blood specimens: isolation, identification, phylogenetic diversity and bioactivity. Phytothe Res 2011, 25:1189-1195.
• [37]Jiang DF, Ma P, Yang J, Wang X, Xu K, Huang Y, Chen S: Formation of blood resin in abiotic Dracaena cochinchinensis inoculated with Fusarium 9568D. Ying Yong Sheng Tai Xue Bao 2003, 14:477-478.
• [38]Li LY, Ding Y, Groth I, Menzel KD, Peschel G, Voigt K, Deng ZW, Sattler I, Lin WH: Pyrrole and indole alkaloids from an endophytic Fusarium incarnatum (HKI00504) isolated from the mangrove plant Aegiceras corniculatum. Bioorg Med Chem 2008, 16:7894-7899.
• [39]Li XX, Pan XR, Wu LL, Zhang HL: Study on the fungistatic and promoter action of Ophiopogon japonicus (Thunb.) for E. Coli using microcalorimetric method. J Shandong University of TCM 2001, 4:307-309.
• [40]Liu CH, Liu TT, Yuan FF, Gu YC: Isolating endophytic fungi from evergreen plants and determining their antifungal activities. Afr J Microbiol Res 2010, 4:2243-2248.
• [41]Sean FB, Jon C: CR377, a New Pentaketide Antifungal Agent Isolated from an Endophytic Fungus. J Nat Prod 2000, 3:1447-1448.
• [42]Lee JC, Lobkovsky E, Nathan BP, Strobel G, Clardy J: Subglutinols a and B: immunosuppressive compounds from the endophytic fungus fusarium subglutinans. J Org Chem 1995, 60:7076-7077.
• [43]Wang QX, Li SF, Zhao F, Dai HQ, Bao B, Ding R, Gao H, Zhang LX, Wen HA, Liu HW: Chemical constituents from endophytic fungus Fusarium oxysporum. Fitoterapia 2011, 82:777-781.