期刊论文详细信息
BMC Anesthesiology
Effect of propofol on microRNA expression in rat primary embryonic neural stem cells
Research Article
Zaisheng Qin1  Quan Zhou1  Jun Fan1  Tao Tao1 
[1] Department of Anesthesiology, Nan Fang Hospital, Southern Medical University, Guangzhou, Guangdong, China;
关键词: Propofol;    microRNA;    Neural stem cells;    Neurogenesis;   
DOI  :  10.1186/s12871-016-0259-1
 received in 2016-01-20, accepted in 2016-09-29,  发布年份 2016
来源: Springer
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【 摘 要 】

BackgroundPropofol is a widely used intravenous anesthetic that is well-known for its protective effect in various human and animal disease models. However, the effects of propofol on neurogenesis, especially on the development of neural stem cells (NSCs), remains unknown. Related microRNAs may act as important regulators in this process.MethodsPublished Gene Expression Omnibus (GEO) DataSets related to propofol were selected and re-analyzed to screen neural development-related genes and predict microRNA (miRNA) expression using bioinformatic methods. Screening of the genes and miRNAs was then validated by qRT-PCR analysis of propofol-treated primary embryonic NSCs.ResultsFour differentially expressed mRNAs were identified in the screen and 19 miRNAs were predicted based on a published GEO DataSet. Two of four mRNAs and four of 19 predicted miRNAs were validated by qRT-PCR analysis of propofol-treated NSCs. Rno-miR-19a (Rno, Rattus Norvegicus) and rno-miR-137, and their target gene EGR2, as well as rno-miR-19b-2 and rno-miR-214 and their target gene ARC were found to be closely related to neural developmental processes, including proliferation, differentiation, and maturation of NSCs.ConclusionPropofol influences miRNA expression; however, further studies are required to elucidate the mechanism underlying the effects of propofol on the four miRNAs and their target genes identified in this study. In particular, the influence of propofol on the entire development process of NSCs remains to be clarified.

【 授权许可】

CC BY   
© The Author(s). 2016

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