Inhibitory effects of Stemona tuberosa on lung inflammation in a subacute cigarette smoke-induced mouse model

BMC Complementary and Alternative Medicine

Research Article

Hyeonhoon Lee¹  Young Pyo Jang²  Eun Young Chung²  Hyunsu Bae³  Kyung-Hwa Jung³  Soojin Park³  Yun-Seo Kil⁴  Eun-Kyoung Seo⁴ 

[1]Department of Korean Medicine, College of Korean Medicine, Kyung Hee University, #1 Hoeki-dongDongdaemoon-gu, 130-701, Seoul, Republic of Korea

[2]Department of Life and Nanopharmaceutical Science, College of Pharmacy, Kyung Hee University, #1 Hoeki-dongDongdaemoon-gu, 130-701, Seoul, Republic of Korea

[3]Department of Physiology, College of Korean Medicine, Kyung Hee University, #1 Hoeki-dongDongdaemoon-gu, 130-701, Seoul, Republic of Korea

[4]Global Top5 Research Program, College of Pharmacy, Ewha Womans University, 120-750, Seoul, South Korea

关键词: CS;    Stemona tuberosa;    BALF;    COPD;    TNF-α;    IL-6;   

DOI  :  10.1186/1472-6882-14-513

 received in 2014-03-26, accepted in 2014-12-16,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundStemona tuberosa has long been used in Korean and Chinese medicine to ameliorate various lung diseases such as pneumonia and bronchitis. However, it has not yet been proven that Stemona tuberosa has positive effects on lung inflammation.MethodsStemona tuberosa extract (ST) was orally administered to C57BL/6 mice 2 hr before exposure to CS for 2 weeks. Twenty-four hours after the last CS exposure, mice were sacrificed to investigate the changes in the expression of cytokines such as tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6), chemokines such as keratinocyte-derived chemokine (KC) and inflammatory cells such as macrophages, neutrophils, and lymphocytes from bronchoalveolar lavage fluid (BALF). Furthermore, we compared the effect of ST on lung tissue morphology between the fresh air, CS exposure, and ST treatment groups.ResultsST significantly decreased the numbers of total cells, macrophages, neutrophils, and lymphocytes in the BALF of mice that were exposed to CS. Additionally, ST reduced the levels of cytokines (TNF-α, IL-6) and the tested chemokine (KC) in BALF, as measured by enzyme-linked immunosorbent assay (ELISA). We also estimated the mean alveolar airspace (MAA) via morphometric analysis of lung tissues stained with hematoxylin and eosin (H&E). We found that ST inhibited the alveolar airspace enlargement induced by CS exposure. Furthermore, we observed that the lung tissues of mice treated with ST showed ameliorated epithelial hyperplasia of the bronchioles compared with those of mice exposed only to CS.ConclusionsThese results indicate that Stemona tuberosa has significant effects on lung inflammation in a subacute CS-induced mouse model. According to these outcomes, Stemona tuberosa may represent a novel therapeutic herb for the treatment of lung diseases including COPD.

【 授权许可】

Unknown   
© Lee et al.; licensee BioMed Central. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.