Increasing effect of Tangzhiqing formula on IRS-1-dependent PI3K/AKT signaling in muscle

BMC Complementary and Alternative Medicine

Research Article

Yanlin Wu¹  Tao Wang¹  Jian Li¹  Qian Qian²  Xuefeng Liu²  Yating An³  Jing Gao³  Yi Zhang³ 

[1] Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshanxi Road, 300193, Nankai District, Tianjin, China;Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Tianjin University of Traditional Chinese Medicine, Ministry of Education, 312 Anshanxi Road, 300193, Nankai District, Tianjin, China;Tianjin State Key Laboratory of Modern Chinese Medicine, 312 Anshanxi Road, 300193, Nankai District, Tianjin, China;

关键词: IRS-1-dependent PI3K signaling pathway;    Insulin resistance;    Paeonia lactiflora;    Morus alba;    Nelumbo nucifera;    Salvia miltiorrhiza;    Crataegus pinnatifida;   

DOI  :  10.1186/1472-6882-14-198

 received in 2013-11-06, accepted in 2014-06-19,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundTangzhiqing fomula (TZQ-F), the mixture of Red Paeony root, Mulberry leaf, Lotus leaf, Danshen root and Hawthorn leaf, regulates the abnormal glucose and lipids in prediabetic patients. In this study, we focus on the mechanism of TZQ-F and its fractions on glucose metabolism.MethodsAfter orally administration of TZQ-F for 4 weeks in KK-Ay mice, we dissected out the liver and muscle, and employed PCR and western blotting to screening the PI3K/AKT pathway. The following PI3K/AKT signaling pathway were performed in L-6 myotube and HepG2 cells.ResultsIn the liver of KK-Ay mice, no significance was observed on PI3K, AKT and their phosphorylation between TZQ-F and controls , while, in the muscle, up-regulation of PI3K, AKT, Glycogen synthase (GYS) and their phosphorylation type, as well as GluT4, was deteced in TZQ-F. In HepG2 cells, TZQ-F increased IRS-2 by 10 folds, without interrupting AKT, IRS-1 and GluT4. In L-6 myotube cells, TZQ-F and its fractions treatment significantly increased IRS-1 and AKT at mRNA level.ConclusionTZQ-F prevents pre-diabetes through increasing effect on IRS-1-dependent PI3K/AKT signaling pathway in muscle.

【 授权许可】

Unknown   
© Gao et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.