Increasing effect of Tangzhiqing formula on IRS-1-dependent PI3K/AKT signaling in muscle

BMC Complementary and Alternative Medicine

Tao Wang¹  Yi Zhang²  Yanlin Wu¹  Qian Qian³  Xuefeng Liu³  Yating An²  Jian Li¹  Jing Gao² 

[1] Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshanxi Road, Nankai District, Tianjin 300193, China;Tianjin State Key Laboratory of Modern Chinese Medicine, 312 Anshanxi Road, Nankai District, Tianjin 300193, China;Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Tianjin University of Traditional Chinese Medicine, Ministry of Education, 312 Anshanxi Road, Nankai District, Tianjin 300193, China

关键词: Crataegus pinnatifida;    Salvia miltiorrhiza;    Nelumbo nucifera;    Morus alba;    Paeonia lactiflora;    Insulin resistance;    IRS-1-dependent PI3K signaling pathway;   

Others  :  1220090
DOI  :  10.1186/1472-6882-14-198

 received in 2013-11-06, accepted in 2014-06-19,  发布年份 2014

【 摘 要 】

Background

Tangzhiqing fomula (TZQ-F), the mixture of Red Paeony root, Mulberry leaf, Lotus leaf, Danshen root and Hawthorn leaf, regulates the abnormal glucose and lipids in prediabetic patients. In this study, we focus on the mechanism of TZQ-F and its fractions on glucose metabolism.

Methods

After orally administration of TZQ-F for 4 weeks in KK-A^y mice, we dissected out the liver and muscle, and employed PCR and western blotting to screening the PI3K/AKT pathway. The following PI3K/AKT signaling pathway were performed in L-6 myotube and HepG2 cells.

Results

In the liver of KK-A^y mice, no significance was observed on PI3K, AKT and their phosphorylation between TZQ-F and controls , while, in the muscle, up-regulation of PI3K, AKT, Glycogen synthase (GYS) and their phosphorylation type, as well as GluT4, was deteced in TZQ-F. In HepG2 cells, TZQ-F increased IRS-2 by 10 folds, without interrupting AKT, IRS-1 and GluT4. In L-6 myotube cells, TZQ-F and its fractions treatment significantly increased IRS-1 and AKT at mRNA level.

Conclusion

TZQ-F prevents pre-diabetes through increasing effect on IRS-1-dependent PI3K/AKT signaling pathway in muscle.

【 授权许可】

   
2014 Gao et al.; licensee BioMed Central Ltd.

【 图 表 】

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