Anti-emetic mechanisms of zingiber officinale against cisplatin induced emesis in the pigeon; behavioral and neurochemical correlates

BMC Complementary and Alternative Medicine

Research Article

Muhammad Ayaz¹  Gowhar Ali²  Fazal Subhan²  Sami Ullah²  Rehmat Shah³  Ihsan Ullah⁴  Ikram Ul Haq⁵ 

[1] Department of Pharmacy, University of Malakand, Chakdara, Dir Lower, Pakistan;Department of Pharmacy, University of Peshawar, Peshawar, Pakistan;Department of Pharmacy, University of Peshawar, Peshawar, Pakistan;Hospital Pharmacist, Khyber Teaching Hospital, Peshawar, Pakistan;Department of Pharmacy, University of Swabi, Swabi, Pakistan;Department of Pharmacy, University of Peshawar, Peshawar, Pakistan;National Institute of Health, Islamabad, Pakistan;

关键词: Cisplatin;    Emesis;    Zingiber officinale;    Pigeon;    Serotonin;   

DOI  :  10.1186/s12906-015-0556-0

 received in 2014-06-26, accepted in 2015-02-18,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundZingiber officinale (ZO, family Zingiberaceae) has been reported for its antiemetic activity against cancer chemotherapy induced emesis in animal models and in clinics. Current study was designed to investigate ZO for potential usefulness against cisplatin induced vomiting in pigeon and its effects on central and peripheral neurotransmitters involved in the act of vomiting.MethodsZingiber officinale acetone fraction (ZO-ActFr) was investigated for attenuation of emesis induced by cisplatin in healthy pigeons. Neurotransmitters DA, 5HT and their metabolites DOPAC, HVA and 5HIAA were analyzed using High Performance Liquid Chromatography system coupled with electrochemical detector in area postrema, brain stem and intestine. Antiemetic effect of ZO-ActFr was correlated with central and intestinal neurotransmitters levels in pigeon.ResultsCisplatin (7 mg/kg i.v.) induced emesis without lethality upto the observation period. ZO-ActFr (25, 50 & 100 mg/kg) attenuated cisplatin induced emesis ~ 44.18%, 58.13% (P < 0.05) and 27.9%, respectively; the reference drug, metoclopramide (MCP; 30 mg/kg), produced ~ 48.83% reduction (P < 0.05). ZO-ActFr reduced (P < 0.05 - 0.001) 5-hydroxytryptamine (5HT) concentration in the area postrema, brain stem and intestine at 3rd hour of cisplatin administration, while at the 18th hour ZO treatments attenuated the dopamine upsurge (P < 0.001) caused by cisplatin in the area postrema and 5HT concentration (P < 0.01 - 0.001) in the brain stem and intestine. ZO treatments alone did not altered the basal neurotransmitters and their metabolites in the brain areas and intestine.ConclusionThe behavioral study verify the antiemetic profile of ZO against cisplatin induced emesis in the pigeon, where central and peripheral neural evidences advocate the involvement of serotonergic mechanism at initial time point (3rd hr), while the later time point (18th hr) is associated with serotonergic and dopaminergic component in the mediation of its antiemetic effect.

【 授权许可】

CC BY   
© Ullah et al.; licensee BioMed Central. 2015