Cultivable gut bacteria provide a pathway for adaptation of Chrysolina herbacea to Mentha aquatica volatiles

BMC Plant Biology

Research Article

Graziano Pizzolante¹  Salvatore M. Tredici¹  Davide Vergara¹  Pietro Alifano¹  Simon A. Zebelo²  Luigi Del Giudice³  Paola Pontieri³  Patrizia Rubiolo⁴  Carlo Bicchi⁴  Chiara Cordero⁴  Massimo E. Maffei⁵  Andrea Capuzzo⁵  Chidananda N. Kanchiswamy⁶ 

[1] Department of Biological and Environmental Sciences and Technologies, University of Salento, via Monteroni 165, 73100, Lecce, Italy;Department of Natural Sciences, University of Maryland Eastern Shore, 1117 Trigg Hall, 21853, Princess Anne, MD, USA;Dipartimento di Biologia, Sezione di Igiene, Institute of Biosciences and Bioresources-UOS Portici (IBBR-UOS Portici), CNR, Portici (NA) c/o, 80134, Naples, Italy;Dipartimento di Scienza e Tecnologia del Farmaco, Università di Torino, Via Pietro Giuria n°9, 10125, Torino, Italy;Dipartimento di Scienze della Vita e Biologia dei Sistemi, Università di Torino, Via Quarello 15/A, 10135, Torino, Italy;Research and Innovation Centre Genomics and Biology of Fruit Crop Department, Fondazione Edmund Mach (FEM), Istituto Agrario San Michele (IASMA), Via Mach 1, 38010, San Michele all’Adige, TN, Italy;

关键词: Mentha aquatica;    Chrysolina herbacea;    Terpenoids;    Insect pheromones;    Gut microbial community;    Microbial organic volatile compounds;    Antimicrobial compounds;   

DOI  :  10.1186/s12870-017-0986-6

 received in 2016-03-03, accepted in 2017-01-24,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundA chemical cross-talk between plants and insects is required in order to achieve a successful co-adaptation. In response to herbivory, plants produce specific compounds, and feeding insects respond adequately7 to molecules produced by plants. Here we show the role of the gut microbial community of the mint beetle Chrysolina herbacea in the chemical cross-talk with Mentha aquatica (or watermint).ResultsBy using two-dimensional gas chromatography–mass spectrometry we first evaluated the chemical patterns of both M. aquatica leaf and frass volatiles extracted by C. herbacea males and females feeding on plants, and observed marked differences between males and females volatiles. The sex-specific chemical pattern of the frass paralleled with sex-specific distribution of cultivable gut bacteria. Indeed, all isolated gut bacteria from females belonged to either α- or γ-Proteobacteria, whilst those from males were γ-Proteobacteria or Firmicutes. We then demonstrated that five Serratia marcescens strains from females possessed antibacterial activity against bacteria from males belonging to Firmicutes suggesting competition by production of antimicrobial compounds. By in vitro experiments, we lastly showed that the microbial communities from the two sexes were associated to specific metabolic patterns with respect to their ability to biotransform M. aquatica terpenoids, and metabolize them into an array of compounds with possible pheromone activity.ConclusionsOur data suggest that cultivable gut bacteria of Chrysolina herbacea males and females influence the volatile blend of herbivory induced Mentha aquatica volatiles in a sex-specific way.

【 授权许可】

CC BY   
© The Author(s). 2017