In vitro and in vivo reduction of post-prandial blood glucose levels by ethyl alcohol and water Zingiber mioga extracts through the inhibition of carbohydrate hydrolyzing enzymes

BMC Complementary and Alternative Medicine

Research Article

Sung-Hoon Jo¹  Cha-Young Cho¹  Emmanouil Apostolidis¹  Kyoung-Soo Ha¹  Jung-Yoon Lee²  Young-In Kwon² 

[1] Department of Chemistry and Food Science, Framingham State University, 01701, Framingham, MA, USA;Department of Food and Nutrition, Hannam University, 305-811, Daejeon, South Korea;

关键词: α-glucosidase;    Sucrase;    Anti-hyperglycemia;    Blood glucose;    Oxygen radical absorbance capacity;    Zingiber mioga;   

DOI  :  10.1186/s12906-016-1090-4

 received in 2015-11-22, accepted in 2016-03-19,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundType 2 diabetes is a serious problem for developed and developing countries. Prevention of prediabetes progression to type 2 diabetes with the use of natural products appears to be a cost-effective solution. Zingiber mioga has been used as a traditional food in Asia. Recent research has reported the potential health benefits of Zingiber mioga, but the blood glucose reducing effect has not been yet evaluated.MethodsIn this study Zingiber mioga extracts (water and ethanol) were investigated for their anti-hyperglycemic and antioxidant potential using both in vitro and animal models. The in vitro study evaluated the total phenolic content, the oxygen radical absorbance capacity (ORAC) and the inhibitory effect against carbohydrate hydrolyzing enzymes (porcine pancreatic α-amylase and rat intestinal sucrase and maltase) of both Zingiber mioga extracts. Also, the extracts were evaluated for their in vivo post-prandial blood glucose reducing effect using SD rat and db/db mice models.ResultsOur findings suggest that the ethanol extract of Zingiber mioga (ZME) exhibited the higher sucrase and maltase inhibitory activity (IC50, 3.50 and 3.13 mg/mL) and moderate α-amylase inhibitory activity (IC50, >10 mg/mL). Additionally, ZME exhibited potent peroxyl radical scavenging linked antioxidant activity (0.53/TE 1 μM). The in vivo study using SD rat and db/db mice models also showed that ZME reduces postprandial increases of blood glucose level after an oral administration of sucrose by possibly acting as an intestinal α-glucosidase inhibitor (ZME 0.1 g/kg 55.61 ± 13.24 mg/dL)ConclusionThe results indicate that Zingiber mioga extracts exhibited significant in vitro α-glucosidase inhibition and antioxidant activity. Additionally, the tested extracts demonstrated in vivo anti-hyperglycemic effects using SD rat and db/db mice models. Our findings provide a strong rationale for the further evaluation of Zingiber mioga for the potential to contribute as a useful dietary strategy to manage postprandial hyperglycemia.

【 授权许可】

CC BY   
© Jo et al. 2016