Timosaponin-BII inhibits the up-regulation of BACE1 induced by Ferric Chloride in rat retina

BMC Complementary and Alternative Medicine

Research Article

Dan Chen¹  Kun Xiong¹  Lei Shang¹  Hui Wang¹  Chun-Ling Fan¹  Shuang Chen¹  Ju-Fang Huang¹  Meng-Qi Zhang²  Pei Liu³ 

[1] Department of Anatomy and Neurobiology, School of Basic Medical Sciences, Central South University, 410013, Changsha, Hunan, China;Eight-year Clinical Medicine Doctor Program, Grade 2006, Central South University Xiangya School of Medicine, 410013, Changsha, Hunan, China;Five-year Medicine Program, Grade 2009, Central South University Xiangya School of Medicine, 410013, Changsha, Hunan, China;

关键词: Timosaponin-BII;    Oxidative stress;    BACE1;    Retina;    Aβ;   

DOI  :  10.1186/1472-6882-12-189

 received in 2012-06-29, accepted in 2012-10-18,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundOur previous studies indicated that oxidative stress up-regulated the expression of β-amyloid precursor protein cleavage enzyme-1 (BACE1) in rat retina. Pharmacological reports have shown Timosaponin-BII, a purified extract originating from Chinese medical herb Rhizoma Anemarrhenae, is characterized as an antioxidant. Our present study aimed to determine whether Timosaponin-BII affected the expression of BACE1, β-amyloid precursor protein cleavage production of Aβ1-40 and β-C-terminal fragment (β-CTF) in rat retina, which were pre-treated with the oxidizing agent (solution of FeCl3).ResultsFew distinctions of BACE1 distribution were observed among all groups (normal control group, model group, Timosaponin-BII treated and vehicle control groups). Rat retinas in model group and vehicle control group manifested an apparent up-regulation of BACE1 expression. Meanwhile, the level of malonaldehyde (MDA), Aβ1-40 and β-CTF were increased. However, when comparing with the vehicle control group, the retinas in Timosaponin-BII treated group showed significantly less BACE1 (p<0.05) and accumulated less Aβ1-40 or β-CTF (p<0.05). It also showed significantly decreased level of MDA (p<0.05) and prolonged partial thromboplastin time (p<0.05).ConclusionOur data suggested that Timosaponin-BII remarkably inhibited the up-regulation of BACE1 and reduced the over-production of β-CTF and Aβ in rat retina, which was induced by FeCl3. The mechanism of Timosaponin-BII on BACE1 expression may be related to its antioxidant property.

【 授权许可】

Unknown   
© Huang et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.