Protective effects of biochanin A on articular cartilage: in vitro and in vivo studies

BMC Complementary and Alternative Medicine

Research Article

Hui-ming Zhong¹  Ding-Qian Wu²  Li Ba²  Qian-hai Ding³ 

[1] Department of Emergency Medicine, The Second Affiliated Hospital of School of Medicine, Zhejiang University, Jie Fang Road 88#, 310009, Hangzhou, People’s Republic of China;Department of Emergency Medicine, The Second Affiliated Hospital of School of Medicine, Zhejiang University, Jie Fang Road 88#, 310009, Hangzhou, People’s Republic of China;Research Institute of Emergency Medicine, Jie Fang Road 88#, 310009, Hangzhou, People’s Republic of China;Department of Orthopedic Surgery, The Second Affiliated Hospital of School of Medicine, Zhejiang University, Jie Fang Road 88#, 310009, Hangzhou, People’s Republic of China;

关键词: Biochanin A;    Osteoarthritis;    Chondroprotection;    Interleukin-1beta;    Matrix metalloproteinases;    Anterior cruciate ligament transection (ACLT);   

DOI  :  10.1186/1472-6882-14-444

 received in 2014-04-23, accepted in 2014-10-29,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundIncreased production of matrix metalloproteinases (MMPs) is closely related to the progression of osteoarthritis (OA). The present study was performed to investigate the potential value of biochanin A in inhibition of MMP expression in both rabbit chondrocytes and an animal model of OA.MethodsMTT assay was performed to assess chondrocyte survival in monolayers. The mRNA and protein expression of MMPs (including MMP-1, MMP-3, and MMP-13) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in interleukin-1 < beta > (IL-1β)-induced rabbit chondrocytes were determined by quantitative real-time PCR and enzyme-linked immunosorbent assay (ELISA), respectively. The involvement of the NF-kappaB (NF-κB) pathway activated by IL-1β was determined by western blotting. The in vivo effects of biochanin A were evaluated by intra-articular injection in an experimental OA rabbit model induced by anterior cruciate ligament transection (ACLT).ResultsBiochanin A downregulated the expression of MMPs and upregulated TIMP-1 at both the mRNA and protein levels in IL-1β-induced chondrocytes in a dose-dependent manner. In addition, IL-1β-induced activation of NF-κB was attenuated by biochanin A, as determined by western blotting. Moreover, biochanin A decreased cartilage degradation as determined by both morphological and histological analyses in vivo.ConclusionsTaken together, these findings suggest that biochanin A may be a useful agent in the treatment and prevention of OA.

【 授权许可】

Unknown   
© Wu et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.