Protective effects of biochanin A on articular cartilage: in vitro and in vivo studies

BMC Complementary and Alternative Medicine

Li Ba¹  Qian-hai Ding³  Hui-ming Zhong²  Ding-Qian Wu¹ 

[1] Research Institute of Emergency Medicine, Jie Fang Road 88#, Hangzhou 310009, People’s Republic of China;Department of Emergency Medicine, The Second Affiliated Hospital of School of Medicine, Zhejiang University, Jie Fang Road 88#, Hangzhou 310009, People’s Republic of China;Department of Orthopedic Surgery, The Second Affiliated Hospital of School of Medicine, Zhejiang University, Jie Fang Road 88#, Hangzhou 310009, People’s Republic of China

关键词: Anterior cruciate ligament transection (ACLT);    Matrix metalloproteinases;    Interleukin-1beta;    Chondroprotection;    Osteoarthritis;    Biochanin A;   

Others  :  1085475
DOI  :  10.1186/1472-6882-14-444

 received in 2014-04-23, accepted in 2014-10-29,  发布年份 2014

【 摘 要 】

Background

Increased production of matrix metalloproteinases (MMPs) is closely related to the progression of osteoarthritis (OA). The present study was performed to investigate the potential value of biochanin A in inhibition of MMP expression in both rabbit chondrocytes and an animal model of OA.

Methods

MTT assay was performed to assess chondrocyte survival in monolayers. The mRNA and protein expression of MMPs (including MMP-1, MMP-3, and MMP-13) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in interleukin-1 < beta > (IL-1β)-induced rabbit chondrocytes were determined by quantitative real-time PCR and enzyme-linked immunosorbent assay (ELISA), respectively. The involvement of the NF-kappaB (NF-κB) pathway activated by IL-1β was determined by western blotting. The in vivo effects of biochanin A were evaluated by intra-articular injection in an experimental OA rabbit model induced by anterior cruciate ligament transection (ACLT).

Results

Biochanin A downregulated the expression of MMPs and upregulated TIMP-1 at both the mRNA and protein levels in IL-1β-induced chondrocytes in a dose-dependent manner. In addition, IL-1β-induced activation of NF-κB was attenuated by biochanin A, as determined by western blotting. Moreover, biochanin A decreased cartilage degradation as determined by both morphological and histological analyses in vivo.

Conclusions

Taken together, these findings suggest that biochanin A may be a useful agent in the treatment and prevention of OA.

【 授权许可】

   
2014 Wu et al.; licensee BioMed Central Ltd.

【 图 表 】

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