Protective effects of synbiotic diets of Bacillus coagulans, Lactobacillus plantarum and inulin against acute cadmium toxicity in rats

BMC Complementary and Alternative Medicine

Research Article

Saeed Nazifi¹  Dornoush Jafarpour²  Seyed Shahram Shekarforoush²  Hamid Reza Ghaisari²  Mohammad Hadi Eskandari³  Javad Sajedianfard⁴ 

[1] Department of Clinical Pathology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran;Department of Food Hygiene and Public Health, School of Veterinary Medicine, Shiraz University, Shiraz, Iran;Department of Food Science and Technology, College of Agriculture, Shiraz University, Shiraz, Iran;Department of Physiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran;

关键词: Cadmium;    Lactobacillus plantarum;    Bacillus Coagulans;    Inulin;    Oxidative stress;   

DOI  :  10.1186/s12906-017-1803-3

 received in 2016-09-24, accepted in 2017-05-22,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundCadmium is a heavy metal that causes oxidative stress and has toxic effects in humans. The aim of this study was to investigate the influence of two probiotics along with a prebiotic in preventing the toxic effects of cadmium in rats.MethodsTwenty-four male Wistar rats were randomly divided into four groups namely control, cadmium only, cadmium along with Lactobacillus plantarum (1 × 109 CFU/day) and inulin (5% of feedstuff) and cadmium along with Bacillus coagulans (1 × 109 spore/day) and inulin (5% of feedstuff). Cadmium treated groups received 200 μg/rat/day CdCl2 administered by gavage. During the 42-day experimental period, they were weighed weekly. For evaluation of changes in oxidative stress, the levels of some biochemicals and enzymes of serum including SOD, GPX, MDA, AST, ALT, total bilirubin, BUN and creatinine, and also SOD level of livers were measured at day 21 and 42 of treatment. The cadmium content of kidney and liver was determined by using atomic absorption mass spectrophotometry. Data were analyzed using analysis of variance (ANOVA) followed by Duncan’s post hoc test.ResultsTreatment of cadmium induced rats with synbiotic diets significantly improved the liver enzymes and biochemical parameters that decreased AST, ALT, total bilirubin, BUN and metal accumulation in the liver and kidney and increased body weight, serum and liver SOD values in comparison with the cadmium-treated group. No significant differences were observed with MDA and GPX values between all groups (p > 0.05).ConclusionsThis study showed that synbiotic diets containing probiotics (L. plantarum and B. coagulans) in combination with the prebiotic (inulin) can reduce the level of cadmium in the liver and kidney, preventing their damage and recover antioxidant enzymes in acute cadmium poisoning in rat.

【 授权许可】

CC BY   
© The Author(s). 2017