Correlates of the molecular vaginal microbiota composition of African women

BMC Infectious Diseases

Research Article

Hanneke Borgdorff¹  Rita Verhelst²  Raju Gautam³  Janneke HHM van de Wijgert⁴  Jordan K Kyongo⁵  Vicky Jespers⁵  Tania Crucitti⁵  Liselotte Hardy⁵  Joris Menten⁵  Mary Mwaura⁶  Suzanna C Francis⁷  Gilles Ndayisaba⁸  Frank Schuren⁹  Evgeni Tsivtsivadze⁹  Sinead Delany-Moretlwe^1,10 

[1] Amsterdam Institute for Global Health and Development and Academic Medical Center, Amsterdam, The Netherlands;Ghent University, International Centre for Reproductive Health, Ghent, Belgium;Institute of Infection and Global Health, University of Liverpool, Liverpool, UK;Institute of Infection and Global Health, University of Liverpool, Liverpool, UK;Rinda Ubuzima, Kigali, Rwanda;Department of Clinical Infection, Immunology and Microbiology, Institute of Infection and Global Health, University of Liverpool, Ronald Ross Building, West Derby Street, L69 7BE, Liverpool, UK;Institute of Tropical Medicine, Antwerp, Belgium;International Centre for Reproductive Health Kenya, Mombasa, Kenya;London School for Hygiene and Tropical Medicine, MRC Tropical Epidemiology Group, London, UK;Rinda Ubuzima, Kigali, Rwanda;TNO Quality of Life, Zeist, The Netherlands;Wits Reproductive Health and HIV Research Institute, Witwatersrand University, Johannesburg, South Africa;

关键词: Bacterial vaginosis;    Vaginal microbiome;    Vaginal microbiota;    Lactobacillus;    Candidiasis;    Sexually transmitted infections;    Urinary tract infections;    Women;    HIV;    Africa;   

DOI  :  10.1186/s12879-015-0831-1

 received in 2014-08-19, accepted in 2015-02-11,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundSociodemographic, behavioral and clinical correlates of the vaginal microbiome (VMB) as characterized by molecular methods have not been adequately studied. VMB dominated by bacteria other than lactobacilli may cause inflammation, which may facilitate HIV acquisition and other adverse reproductive health outcomes.MethodsWe characterized the VMB of women in Kenya, Rwanda, South Africa and Tanzania (KRST) using a 16S rDNA phylogenetic microarray. Cytokines were quantified in cervicovaginal lavages. Potential sociodemographic, behavioral, and clinical correlates were also evaluated.ResultsThree hundred thirteen samples from 230 women were available for analysis. Five VMB clusters were identified: one cluster each dominated by Lactobacillus crispatus (KRST-I) and L. iners (KRST-II), and three clusters not dominated by a single species but containing multiple (facultative) anaerobes (KRST-III/IV/V). Women in clusters KRST-I and II had lower mean concentrations of interleukin (IL)-1α (p < 0.001) and Granulocyte Colony Stimulating Factor (G-CSF) (p = 0.01), but higher concentrations of interferon-γ-induced protein (IP-10) (p < 0.01) than women in clusters KRST-III/IV/V. A lower proportion of women in cluster KRST-I tested positive for bacterial sexually transmitted infections (STIs; ptrend = 0.07) and urinary tract infection (UTI; p = 0.06), and a higher proportion of women in clusters KRST-I and II had vaginal candidiasis (ptrend = 0.09), but these associations did not reach statistical significance. Women who reported unusual vaginal discharge were more likely to belong to clusters KRST-III/IV/V (p = 0.05).ConclusionVaginal dysbiosis in African women was significantly associated with vaginal inflammation; the associations with increased prevalence of STIs and UTI, and decreased prevalence of vaginal candidiasis, should be confirmed in larger studies.

【 授权许可】

Unknown   
© Gautam et al.; licensee BioMed Central. 2015. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.