A gallotannin-rich fraction from Caesalpinia spinosa (Molina) Kuntze displays cytotoxic activity and raises sensitivity to doxorubicin in a leukemia cell line

BMC Complementary and Alternative Medicine

Research Article

Luis Miguel Pombo¹  Claudia Patricia Urueña²  Susana Fiorentino²  John Fredy Hernandez²  Diana M Castañeda² 

[1] Grupo de Farmacología Vegetal, Fundación Universitaria Juan N. Corpas, Carrera 111 # 159A61, Bogotá, Colombia;Grupo de Inmunobiología y Biología Celular, Facultad de Ciencias, Pontificia Universidad Javeriana, Carrera 7 N. 43-82 Building 52, Office 608, Bogotá, Colombia;

关键词: Adjuvants;    Gallotannins;    C.spinosa;    Tumor;    Leukemia;   

DOI  :  10.1186/1472-6882-12-38

 received in 2011-09-27, accepted in 2012-04-10,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundEnhancement of tumor cell sensitivity may help facilitate a reduction in drug dosage using conventional chemotherapies. Consequently, it is worthwhile to search for adjuvants with the potential of increasing chemotherapeutic drug effectiveness and improving patient quality of life. Natural products are a very good source of such adjuvants.MethodsThe biological activity of a fraction enriched in hydrolysable polyphenols (P2Et) obtained from Caesalpinia spinosa was evaluated using the hematopoietic cell line K562. This fraction was tested alone or in combination with the conventional chemotherapeutic drugs doxorubicin, vincristine, etoposide, camptothecin and taxol. The parameters evaluated were mitochondrial depolarization, caspase 3 activation, chromatin condensation and clonogenic activity.ResultsWe found that the P2Et fraction induced mitochondrial depolarization, activated caspase 3, induced chromatin condensation and decreased the clonogenic capacity of the K562 cell line. When the P2Et fraction was used in combination with chemotherapeutic drugs at sub-lethal concentrations, a fourfold reduction in doxorubicin inhibitory concentration 50 (IC50) was seen in the K562 cell line. This finding suggested that P2Et fraction activity is specific for the molecular target of doxorubicin.ConclusionsOur results suggest that a natural fraction extracted from Caesalpinia spinosa in combination with conventional chemotherapy in combination with natural products on leukemia cells may increase therapeutic effectiveness in relation to leukemia.

【 授权许可】

Unknown   
© Castañeda et al; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.