Huatuo Zaizao pill promotes functional recovery and neurogenesis after cerebral ischemia-reperfusion in rats

BMC Complementary and Alternative Medicine

Research Article

Sijin Duan¹  Minmin Li¹  Chengwen Lu¹  Fenghua Fu¹  Yan Zou¹  Jianqiao Zhang¹  Lijie Wang¹  Tian Wang¹ 

[1] School of Pharmacy, Key Laboratory of Molecular Pharmacology and Drug Evaluation, Yantai University, 264005, Yantai, People’s Republic of China;Ministry of Education, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Yantai University, 264005, Yantai, People’s Republic of China;

关键词: Huatuo Zaizao pill;    Stroke;    Functional recovery;    Cerebral ischemia-reperfusion;    Neurogenesis;   

DOI  :  10.1186/s12906-016-1516-z

 received in 2016-03-22, accepted in 2016-12-14,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundIschemic stroke is the third leading cause of death in adults worldwide and is the first leading cause of long-term disability. Neurogenesis plays an important role in promoting behavioral recovery after stroke. Huatuo Zaizao pill (HT), a traditional Chinese medicine, has been used clinically in China to promote the rehabilitation after stroke, but the underlying mechanism of action was still unclear. This study is to investigate the effects of HT on the functional recovery in a rat model of cerebral ischemia-reperfusion (I/R) injury, and the potential molecular mechanisms.MethodsRats were randomly divided into sham, model with cerebral I/R injury, or HT-treated groups, then administered orally with vehicle (for the sham and model group) or HT (0.5, 1.0, or 2.0 mg/kg) respectively, for 3 or 7 days. Functional recovery was assessed by cylinder test, beam walking test, and adhesive test. Neurogenesis was investigated by double immunofluorescence staining for 5-ethynyl-2-deoxyuridine (EdU) and neuronal nuclear protein (NeuN). The proteins of kinase A (PKA), cAMP response element-binding protein (CREB), and brain-derived neurotrophic factor (BDNF) were assayed by western blotting. The level of BDNF mRNA was evaluated by RT-PCR.ResultsCompared with the model group, treatment with HT significantly promoted functional recovery in I/R injured rats (p < 0.05 or p < 0.01). The generation of new neurons was increased in the HT groups. HT treatment for 3 days increased the level of BDNF mRNA in I/R injured rats. Expression of PKA, phosphorylated CREB, and BDNF were significantly (p < 0.05) increased with the 7-day HT treatment.ConclusionsThese results indicated that HT treatment could promote functional recovery after stroke. HT enhanced the expression of BDNF and increased the level of neurogenesis in cerebral I/R animal, which might be associated with the functional recovery.

【 授权许可】

CC BY   
© The Author(s). 2017