Saussurea lappa extract suppresses TPA-induced cell invasion via inhibition of NF-κB-dependent MMP-9 expression in MCF-7 breast cancer cells

BMC Complementary and Alternative Medicine

Research Article

Jeong-Mi Kim¹  Yeon-Ju Park¹  Jin-Ki Hwang¹  Mi-Seong Kim¹  Ha-Rim Kim²  Kang-Beom Kwon³  Young-Rae Lee⁴  Hye-Jung Kim⁵  Yun-Jin Rhee⁶  Byung-Soon Moon⁶  Jong-Min Yun⁶  Sei-Hoon Yang⁷  Do-Gon Ryu⁸  Dong-Sung Lee⁹  Youn-Chul Kim^1,10  Hyuncheol Oh^1,10 

[1] Center for Metabolic Function Regulation, Wonkwang University School of Medicine, 570-749, Iksan, South Korea;Center for Metabolic Function Regulation, Wonkwang University School of Medicine, 570-749, Iksan, South Korea;BK21plus program & Department of Smart Life-Care Convergence, Wonkwang University, Graduate School, #460 Iksan-daero, 570-749, Iksan City, Jeonbuk, South Korea;Center for Metabolic Function Regulation, Wonkwang University School of Medicine, 570-749, Iksan, South Korea;Department of Korean Physiology, Wonkwang University School of Korean Medicine, 570-749, Iksan, South Korea;BK21plus program & Department of Smart Life-Care Convergence, Wonkwang University, Graduate School, #460 Iksan-daero, 570-749, Iksan City, Jeonbuk, South Korea;Center for Metabolic Function Regulation, Wonkwang University School of Medicine, 570-749, Iksan, South Korea;Department of Oral Biochemistry, and Institute of Biomaterials, Implant, School of Dentistry, Wonkwang University, 570-749, Iksan, South Korea;BK21plus program & Department of Smart Life-Care Convergence, Wonkwang University, Graduate School, #460 Iksan-daero, 570-749, Iksan City, Jeonbuk, South Korea;Department of Family Medicine, The Catholic University of Korea, Incheon St. Mary's Hospital, 403-720, Incheon, South Korea;Department of Internal Medicine, College of Korean Medicine, Wonkwang University, #460 Iksan-daero, 570-749, Iksan City, Jeonbuk, South Korea;Department of Internal Medicine, Wonkwang University School of Medicine, 570-749, Iksan, South Korea;Department of Korean Physiology, Wonkwang University School of Korean Medicine, 570-749, Iksan, South Korea;Hanbang Body-Fluid Research Center, Wonkwang University, #460 Iksan-daero, 570-749, Iksan, Jeonbuk, South Korea;Hanbang Body-Fluid Research Center, Wonkwang University, #460 Iksan-daero, 570-749, Iksan, Jeonbuk, South Korea;Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University, #460 Iksan-daero, 570-749, Iksan, Jeonbuk, South Korea;Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University, #460 Iksan-daero, 570-749, Iksan, Jeonbuk, South Korea;

关键词: Saussurea lappa;    MMP-9;    Invasion;    NF-κB;    MCF-7;   

DOI  :  10.1186/1472-6882-14-170

 received in 2013-12-12, accepted in 2014-05-13,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundSaussurea lappa (SL) has been used as a traditional herbal medicine to treat abdominal pain and tenesmus, and has been suggested to possess various biological activities, including anti-tumor, anti-ulcer, anti-inflammatory, anti-viral, and cardiotonic activities. The effect of SL on breast cancer metastasis, however, is unknown. Cell migration and invasion are crucial in neoplastic metastasis. Matrix metalloproteinase-9 (MMP-9), which degrades the extracellular matrix, is a major component in cancer cell invasion.MethodsCell viability was examined by MTT assay, whereas cell motility was measured by invasion assay. Western blot, Real-time PCR, and Zymography assays were used to investigate the inhibitory effects of ESL on matrix metalloproteinase-9 (MMP-9) expression level in MCF-7 cells. EMSA confirmed the inhibitory effects of ESL on DNA binding of NF- κB in MCF-7 cells.ResultsCells threated with various concentrations of Saussurea lappa (ESL) for 24 h. Concentrations of 2 or 4 μM did not lead to a significant change in cell viability or morphology. Therefore, subsequent experiments utilized the optimal non-toxic concentration (2 or 4 μM) of ESL. In this study, we investigated the inhibitory effect of ethanol extract of ESL on MMP-9 expression and cell invasion in 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced MCF-7 cells. ESL inhibited the TPA-induced transcriptional activation of nuclear factor-kappa B (NF-κB). However, this result obtained that ESL did not block the TPA-induced phosphorylation of the kinases: p38, ERK, and JNK. Therefore, ELS-mediated inhibition of TPA-induced MMP-9 expression and cell invasion involves the suppression of NF-kB pathway in MCF-7 cells.ConclusionsThese results indicate that ELS-mediated inhibition of TPA-induced MMP-9 expression and cell invasion involves the suppression of NF-kB pathway in MCF-7 cells. Thus, ESL has potential for controlling breast cancer invasiveness in vitro.

【 授权许可】

Unknown   
© Kim et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.