Essential oils purified from Schisandrae semen inhibits tumor necrosis factor-α-induced matrix metalloproteinase-9 activation and migration of human aortic smooth muscle cells

BMC Complementary and Alternative Medicine

Research Article

Hye Jin Hwang¹  Byung Woo Kim²  Sae Kwang Ku³  Cheol Min Kim⁴  Jin-Woo Jeong⁵  Yung Hyun Choi⁶  Gi-Young Kim⁷  Hae Young Chung⁸  Ki Young Kim⁹  Sung Goo Kim⁹  Joo Wan Kim⁹ 

[1] Anti-Aging Research Center & Blue-Bio Industry RIC, Dongeui University, 614-714, Busan, Republic of Korea;Department of Food and Nutrition, Dongeui University, 614-714, Busan, Republic of Korea;Anti-Aging Research Center & Blue-Bio Industry RIC, Dongeui University, 614-714, Busan, Republic of Korea;Department of Life Science and Biotechnology, Dongeui University, 614-714, Busan, Republic of Korea;Department of Anatomy and Histology, College of Korean Medicine, Daegu Haany University, 712-715, Gyeongsan, Republic of Korea;Department of Biochemistry, Busan National University College of Medicine, 626-870, Yangsan, Republic of Korea;Department of Biochemistry, Dongeui University College of Korean Medicine, 614-052, Busan, Republic of Korea;Department of Biochemistry, Dongeui University College of Korean Medicine, 614-052, Busan, Republic of Korea;Anti-Aging Research Center & Blue-Bio Industry RIC, Dongeui University, 614-714, Busan, Republic of Korea;Laboratory of Immunobiology, Department of Marine Life Sciences, Jeju National University, 690-756, Jeju, Republic of Korea;Molecular Inflammation Research Center for Aging Intervention (MRCA), Department of Pharmacy, Pusan National University, 609-735, Busan, Republic of Korea;Research Institute, Bio-Port Korea INC, Marine Bio-industry Development Center, 619-912, Busan, Republic of Korea;

关键词: Schisandrae semen essential oil;    HASMCs;    Invasion;    MMP-9;    NF-κB;   

DOI  :  10.1186/s12906-015-0523-9

 received in 2014-07-27, accepted in 2015-01-14,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundThe migration of vascular smooth muscle cells from the tunica media to the subendothelial region may be a key event in the development of atherosclerosis after arterial injury. In this study, we investigated the potential mechanisms underlying the anti-atherosclerotic effects of Schisandrae Semen essential oil (SSeo) in human aortic smooth muscle cells (HASMCs).MethodsMetalloproteinase-2/9 (MMP-2/9) activity was evaluated by gelatin zymography and gelatinase activity assay kit. The possible mechanisms underlying SSeo-mediated reduction of by tumor necrosis factor (TNF)-α-induced cell invasion and inhibition of secreted and cytosolic MMP-9 production in HASMCs were investigated.ResultsOur results indicate that SSeo treatment has an inhibitory effect on activation as well as expression of MMP-9 induced by TNF-α in HASMCs in a dose-dependent manner without significant cytotoxicity. SSeo attenuated nuclear translocation of TNF-α-mediated nuclear factor-kappa B (NF-κB) and blocked degradation of the NF-κB inhibitor proteins as well as the production of reactive oxygen species. SSeo also reduced TNF-α-induced production of pro-inflammatory mediators such as nitric oxide and prostaglandin E2 and inhibited inducible nitric oxide synthase and cyclooxygenase-2 expression in HASMCs. Furthermore, the Matrigel migration assay showed that SSeo effectively reduced TNF-α-induced HASMC migration compared with that in the control group.ConclusionsTaken together, these results suggest that SSeo treatment suppresses TNF-α-induced HASMC migration by selectively inhibiting MMP-9 expression, which was associated with suppression of the NF-κB signaling pathway. Taken together, these results suggest that SSeo has putative potential anti-atherosclerotic activity.

【 授权许可】

Unknown   
© Jeong et al.; licensee BioMed Central. 2015. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.