Association between tumour infiltrating lymphocytes, histotype and clinical outcome in epithelial ovarian cancer

BMC Cancer

Research Article

Paul D. P. Pharoah¹  Jennifer Alsop²  James D. Brenton²  Honglin Song²  Marie Mack²  H. Raza Ali³  Mercedes Jiminez-Linan⁴  Fiona R. James⁵ 

[1] Department of Oncology, Department of Public Health and Primary Care, University of Cambridge, Cambridge, UK;Department of Oncology, University of Cambridge, Cambridge, UK;Department of Pathology, CRUK Cambridge Institute, University of Cambridge, Cambridge, UK;Department of Pathology, University of Cambridge, Cambridge, UK;Lancashire Teaching Hospitals Foundation NHS Trust, Lancashire, UK;Department of Oncology, University of Cambridge, Cambridge, UK;

关键词: Tumour infiltrating lymphocytes (TILs);    Epithelial ovarian cancer;    Standardised method;    Histological subtype;    Survival time;   

DOI  :  10.1186/s12885-017-3585-x

 received in 2017-03-05, accepted in 2017-08-22,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundThere is evidence that some ovarian tumours evoke an immune response, which can be assessed by tumour infiltrating lymphocytes (TILs). To facilitate adoption of TILs as a clinical biomarker, a standardised method for their H&E visual evaluation has been validated in breast cancer.MethodsWe sought to investigate the prognostic significance of TILs in a study of 953 invasive epithelial ovarian cancer tumour samples, both primary and metastatic, from 707 patients from the prospective population-based SEARCH study. TILs were analysed using a standardised method based on H&E staining producing a percentage score for stromal and intratumoral compartments. We used Cox regression to estimate hazard ratios of the association between TILs and survival.ResultsThe extent of stromal and intra-tumoral TILs were correlated in the primary tumours (n = 679, Spearman’s rank correlation = 0.60, P < 0.001) with a similar correlation in secondary tumours (n = 224, Spearman’s rank correlation = 0.62, P < 0.001). There was a weak correlation between stromal TIL levels in primary and secondary tumour samples (Spearman’s rank correlation = 0.29, P < 0.001) and intra-tumoral TIL levels in primary and secondary tumour samples (Spearman’s rank correlation = 0.19, P = 0.0094). The extent of stromal TILs differed between histotypes (Pearson chi2 (12d.f.) 54.1, P < 0.0001) with higher levels of stromal infiltration in the high-grade serous and endometriod cases. A significant association was observed for higher intratumoral TIL levels and a favourable prognosis (HR 0.74 95% CI 0.55–1.00 p = 0.047).ConclusionThis study is the largest collection of epithelial ovarian tumour samples evaluated for TILs. We have shown that stromal and intratumoral TIL levels are correlated and that their levels correlate with clinical variables such as tumour histological subtype. We have also shown that increased levels of both intratumoral and stromal TILs are associated with a better prognosis; however, this is only statistically significant for intratumoral TILs. This study suggests that a clinically useful immune prognostic indicator in epithelial ovarian cancer could be developed using this technique.

【 授权许可】

CC BY   
© The Author(s). 2017