Anti-lipoapoptotic effects of Alisma orientalis extract on non-esterified fatty acid-induced HepG2 cells

BMC Complementary and Alternative Medicine

Research Article

Ki-Suk Kim¹  Hyeung-Jin Jang¹  Hyeon-Soo Jeong¹  In-Seung Lee¹  Jiyoung Park¹  Jang-Hoon Lee¹  Young-Hwan Cho¹  Kang-Hoon Kim¹  Yumi Kim²  Yun-Cheol Na³ 

[1] College of Korean Medicine, Institute of Korean Medicine, Kyung Hee University, 1 Heogi-dong, 130-701, Dongdaemun-gu, Seoul, Republic of Korea;College of Korean Medicine, Institute of Korean Medicine, Kyung Hee University, 1 Heogi-dong, 130-701, Dongdaemun-gu, Seoul, Republic of Korea;Western Seoul Center, Korea Basic Science Institute, 150 Bugahyeon-ro, 120-140, Seodaemun-gu, Seoul, Republic of Korea;Western Seoul Center, Korea Basic Science Institute, 150 Bugahyeon-ro, 120-140, Seodaemun-gu, Seoul, Republic of Korea;

关键词: Alisma orientale;    HepG2;    Nonalcoholic fatty liver disease;    De novo lipogenesis;    Lipoapoptosis;    Inflammation;   

DOI  :  10.1186/s12906-016-1181-2

 received in 2015-10-29, accepted in 2016-06-14,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundLiver steatosis was caused by lipid accumulation in the liver. Alisma orientale (AO) is recognized as a promising candidate with therapeutic efficacy for the treatment of nonalcoholic fatty liver disease (NAFLD). HepG2 hepatocyte cell line is commonly used for liver disease cell model.MethodThe HepG2 cells were cultured with the NEFAs mixture (oleic and palmitic acids, 2:1 ratio) for 24 h to induce hepatic steatosis. Then different doses of Alisma orientale extract (AOE) was treated to HepG2 for 24 h. Incubated cells were used for further experiments.ResultsThe AOE showed inhibitory effects on lipid accumulation in the Oil Red O staining and Nile red staining tests with no cytotoxicity at a concentration of 300 μg/mL. Fatty acid synthase (FASN) and acetyl-CoA carboxylase 1 (ACC1) mRNA and protein expression level were down-regulated after AOE treatment. Bcl-2 associated X protein (Bax) and c-Jun N-terminal kinase (JNK) mRNA expression level were decreased as well as p-JNK (activated form of JNK), Bax, cleaved caspase-9, caspase-3 protein expression level. Anti-apopototic B-cell lymphoma 2 (Bcl-2) protein level increased after AOE treatment. In addition, inflammatory protein expression including p-p65, p65, COX-2 and iNOS were inhibited by AOE treatment.ConclusionThe results suggest that AOE has anti-steatosis effects that involve lipogenesis, anti-lipoapoptosis, and anti-inflammation in the NEFA-induced NAFLD pathological cell model.

【 授权许可】

CC BY   
© The Author(s). 2016