Comparative mitochondrial proteomic, physiological, biochemical and ultrastructural profiling reveal factors underpinning salt tolerance in tetraploid black locust (Robinia pseudoacacia L.)

BMC Genomics

Research Article

Pei Lei¹  Xiuli Zhang¹  Guanyu Sun¹  Ximei Ji¹  Fanjuan Meng¹  Wahsoon Chow²  Mu Peng³  Qiuxiang Luo³ 

[1] College of Life Science, Northeast Forestry University, 150040, Harbin, China;College of Life Science, Northeast Forestry University, 150040, Harbin, China;Division of Plant Science, Research School of Biology, The Australian National University, 2601, ACT, Australia;College of Life Science, Northeast Forestry University, 150040, Harbin, China;Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field (SAVER), Ministry of Education, Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, Harbin, China;

关键词: Diploid;    Mitochondria;    Physiological characters proteomics;    Salinity stress;    Robinia pseudoacacia L.;    Ultrastructure;   

DOI  :  10.1186/s12864-017-4038-2

 received in 2016-12-23, accepted in 2017-08-08,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundPolyploidy is an important phenomenon in plants because of its roles in agricultural and forestry production as well as in plant tolerance to environmental stresses. Tetraploid black locust (Robinia pseudoacacia L.) is a polyploid plant and a pioneer tree species due to its wide ranging adaptability to adverse environments. To evaluate the ploidy-dependent differences in leaf mitochondria between diploid and tetraploid black locust under salinity stress, we conducted comparative proteomic, physiological, biochemical and ultrastructural profiling of mitochondria from leaves.ResultsMitochondrial proteomic analysis was performed with 2-DE and MALDI-TOF-MS, and the ultrastructure of leaf mitochondria was observed by transmission electron microscopy. According to 2-DE analysis, 66 proteins that responded to salinity stress significantly were identified from diploid and/or tetraploid plants and classified into 9 functional categories. Assays of physiological characters indicated that tetraploids were more tolerant to salinity stress than diploids. The mitochondrial ultrastructure of diploids was damaged more severely under salinity stress than that of tetraploids.ConclusionsTetraploid black locust possessed more tolerance of, and ability to acclimate to, salinity stress than diploids, which may be attributable to the ability to maintain mitochondrial structure and to trigger different expression patterns of mitochondrial proteins during salinity stress.

【 授权许可】

CC BY   
© The Author(s). 2017