Anti-staphylococcal activities of lysostaphin and LytM catalytic domain

BMC Microbiology

Research Article

Andrej Tarkowski¹  Ing-Marie Jonsson¹  Izabela Sabala²  Matthias Bochtler³ 

[1] Department of Rheumatology and Inflammation Research, University of Gothenburg, Guldhedsgatan 10A, S-413 46, Gothenburg, Sweden;International Institute of Molecular and Cell Biology, Trojdena 4, 02-109, Warsaw, Poland;Max-Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108, 01309, Dresden, Germany;International Institute of Molecular and Cell Biology, Trojdena 4, 02-109, Warsaw, Poland;Max-Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108, 01309, Dresden, Germany;Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5a, 02-106, Warszawa, Poland;

关键词: Peptidoglycan;    Minimal Bactericidal Concentration;    Oxazolone;    Occlude Region;    Cell Wall Preparation;   

DOI  :  10.1186/1471-2180-12-97

 received in 2012-01-05, accepted in 2012-06-06,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundLysostaphin and the catalytic domain of LytM cleave pentaglycine crossbridges of Staphylococcus aureus peptidoglycan. The bacteriocin lysostaphin is secreted by Staphylococcus simulans biovar staphylolyticus and directed against the cell walls of competing S. aureus. LytM is produced by S. aureus as a latent autolysin and can be activated in vitro by the removal of an N-terminal domain and occluding region.ResultsWe compared the efficacies of the lysostaphin and LytM catalytic domains using a newly developed model of chronic S. aureus infected eczema. Lysostaphin was effective, like in other models. In contrast, LytM was not significantly better than control. The different treatment outcomes could be correlated with in vitro properties of the proteins, including proteolytic stability, affinity to cell wall components other than peptidoglycan, and sensitivity to the ionic milieu.ConclusionsAlthough lysostaphin and LytM cleave the same peptide bond in the peptidoglycan, the two enzymes have very different environmental requirements what is reflected in their contrasting performance in mouse eczema model.

【 授权许可】

CC BY   
© Sabala et al.; licensee BioMed Central Ltd. 2012