The relationship between hepatic resistin overexpression and inflammation in patients with nonalcoholic steatohepatitis

BMC Gastroenterology

Research Article

Meng Li¹  Jian Gao²  Chuan Shen³  Cai-Yan Zhao³  Wei-Yan Yu³  Li Zhang³  Wei Wang³  Ya-Dong Wang³  Wei Cao³  Ru Ji³  Hui Sun³ 

[1] Department of Endocrinology and Metabolism, The Third Affiliated Hospital of Hebei Medical University, 139 Ziqiang Road, 050051, Shijiazhuang, China;Department of Hepatobiliary Surgery, The Third Affiliated Hospital of Hebei Medical University, 139 Ziqiang Road, 050051, Shijiazhuang, China;Department of Infectious Disease, The Third Affiliated Hospital of Hebei Medical University, 139 Ziqiang Road, 050051, Shijiazhuang, China;

关键词: Resistin;    Nonalcoholic steatohepatitis;    Nonalcoholic fatty liver disease;    Inflammation;    Adipokine;   

DOI  :  10.1186/1471-230X-14-39

 received in 2013-10-18, accepted in 2014-02-13,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundThe relationship between resistin and non-alcoholic steatohepatitis (NASH) is not clear, some studies claimed that serum resistin levels were associated with neither the presence of NASH nor its severity, others declared that serum resistin was related with inflammation and fibrosis in NASH. Our animal study verified that the distribution of resistin in the liver is correlated with inflammation in NASH. However, there is no pertinent study in humans.MethodsThirty patients with NASH, 28 simple steatosis, and 43 controls were recruited. Blood was collected for resistin, liver chemistries, fasting insulin and some metabolic parameters. Liver histology was scored according to NAFLD activity scoring system. Hepatic resistin expression was examined by real-time polymerase chain reaction, immunohistochemistry. Resistin protein expression was confirmed by western blotting in 13 patients with concomitant NAFLD and gallstone.ResultsSerum resistin was significantly elevated in both NASH and simple steatotic subjects compared with controls (all P < 0.05). Hepatic resistin was significantly increased in NASH patients in both mRNA and protein levels than those in simple steatosis and control subjects (all P < 0.05). Both serum and hepatic resistin had a correlation with obesity, but not with insulin resistance. The distribution of resistin positive cells was predominantly in perisinusoidal cells (such as Kupffer cells and hepatic stellate cells) in human NASH. Multivariate analysis revealed that waist-hip ratio, higher serum triglyceride, and hyperresistinemia were independent factors related to higher grade of steatosis; whereas hepatic resistin and serum cytokeratin predict NASH and severity of liver fibrosis.ConclusionsHepatic resistin overexpression in NASH patients is associated with the severity of liver inflammation and fibrosis. Liver-derived resistin may be involved in the pathogenesis of human NASH.

【 授权许可】

Unknown   
© Shen et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.