Neuroprotective effects of Eucommia ulmoides Oliv. and its bioactive constituent work via ameliorating the ubiquitin-proteasome system

BMC Complementary and Alternative Medicine

Research Article

Meijiao Li¹  Limin Hu¹  Fang Shi¹  Hong Guo¹  Bin Yu¹  Qingqing Liu¹ 

[1] Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, #312 Anshan Xi Road, Nankai District, 300193, Tianjin, China;

关键词: Eucommia ulmoides;    Parkinson’s disease;    Ubiquitin-proteasome system;   

DOI  :  10.1186/s12906-015-0675-7

 received in 2014-09-16, accepted in 2015-05-13,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundParkinson’s disease (PD) is a chronic neurodegenerative disorder characterized by a loss of dopaminergic neurons in the substantia nigra, decreased striatal dopamine levels, and consequent extrapyramidal motor dysfunction. The purpose of this study was to investigate potential in vivo protective effects of Duzhong against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), as well as the bioactive constituents against 1-methyl-4-phenylpyridinium (MPP+) toxicity in vitro.MethodsMale C57BL/6 mice were intraperitoneally administrated five consecutive injections of MPTP every 24 h at a dose of 30 mg/kg to induce an in vivo PD model. Pole and traction tests were performed in mice to evaluate motor deficits and bradykinesia after the final MPTP administration. The striatal levels of dopamine and its metabolites, 3,4-dihydroxyphenylacetic acid and homovanilic acid, were measured using a High-performance liquid chromatography-electrical conductivity detector. To further explore the bioactive constituents and protective mechanisms of Duzhong, seven compounds from Duzhong were tested on MPP+-treated SH-SY5Y cell lines in vitro. A proteasome enzymatic assay and Cell Counting Kit-8 were performed to examine proteasomal activity and cell viability of Duzhong-treated cells, respectively, after exposure to MPP+ and proteasome inhibitor MG132.ResultsDuzhong antagonized the loss of striatal neurotransmitters and relieved the associated anomaly in ambulatory locomotor activity in PD mice after a 3-day pre-treatment of Duzhong crude extract. The five Duzhong compounds attenuated MPP+-induced dysfunction of protease activity and reduced MG132-induced cytotoxicity.ConclusionDuzhong could serve as a potential candidate for PD treatment, and its mechanism involves the amelioration of the ubiquitin-proteasome system.

【 授权许可】

Unknown   
© Guo et al.; licensee BioMed Central. 2015. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.