Genome analysis of Daldinia eschscholtzii strains UM 1400 and UM 1020, wood-decaying fungi isolated from human hosts

BMC Genomics

Research Article

Wai-Yan Yee¹  Kok Wei Lee¹  Chee-Choong Hoh¹  Kee Peng Ng²  Chai Ling Chan²  Su Mei Yew²  Shiang Ling Na²  Yun Fong Ngeow³ 

[1] Codon Genomics S/B, No 26, Jalan Dutamas 7, Taman Dutamas, Balakong, Seri Kembangan, 43200, Selangor Darul Ehsan, Malaysia;Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia;Department of Pre-Clinical Sciences, Faculty of Medicine and Health Sciences, University Tunku Abdul Rahman, Bandar Sungai Long, 43000, Kajang, Selangor Darul Ehsan, Malaysia;

关键词: Genome sequencing;    Daldinia eschscholtzii;    Wood-inhabiting endophyte;    Wood-decaying fungi;   

DOI  :  10.1186/s12864-015-2200-2

 received in 2014-08-28, accepted in 2015-11-10,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundDaldinia eschscholtzii is a wood-inhabiting fungus that causes wood decay under certain conditions. It has a broad host range and produces a large repertoire of potentially bioactive compounds. However, there is no extensive genome analysis on this fungal species.ResultsTwo fungal isolates (UM 1400 and UM 1020) from human specimens were identified as Daldinia eschscholtzii by morphological features and ITS-based phylogenetic analysis. Both genomes were similar in size with 10,822 predicted genes in UM 1400 (35.8 Mb) and 11,120 predicted genes in UM 1020 (35.5 Mb). A total of 751 gene families were shared among both UM isolates, including gene families associated with fungus-host interactions. In the CAZyme comparative analysis, both genomes were found to contain arrays of CAZyme related to plant cell wall degradation. Genes encoding secreted peptidases were found in the genomes, which encode for the peptidases involved in the degradation of structural proteins in plant cell wall. In addition, arrays of secondary metabolite backbone genes were identified in both genomes, indicating of their potential to produce bioactive secondary metabolites. Both genomes also contained an abundance of gene encoding signaling components, with three proposed MAPK cascades involved in cell wall integrity, osmoregulation, and mating/filamentation. Besides genomic evidence for degrading capability, both isolates also harbored an array of genes encoding stress response proteins that are potentially significant for adaptation to living in the hostile environments.ConclusionsOur genomic studies provide further information for the biological understanding of the D. eschscholtzii and suggest that these wood-decaying fungi are also equipped for adaptation to adverse environments in the human host.

【 授权许可】

CC BY   
© Chan et al. 2015