Identification and characterization of Daldinia eschscholtzii isolated from skin scrapings, nails, and blood

PeerJ

article

Kee Peng Ng¹  Chai Ling Chan¹  Su Mei Yew¹  Siok Koon Yeo²  Yue Fen Toh¹  Hong Keat Looi¹  Shiang Ling Na¹  Kok Wei Lee³  Wai-Yan Yee³  Chee Sian Kuan¹ 

[1] Department of Medical Microbiology, University of Malaya;School of Biosciences, Taylor’s University, Lakeside Campus;Codon Genomics

关键词: Daldinia eschscholtzii;    Internal transcribed spacer;    Plylogenetic analysis;   

DOI  :  10.7717/peerj.2637

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundDaldinia eschscholtzii is a filamentous wood-inhabiting endophyte commonly found in woody plants. Here, we report the identification and characterization of nine D. eschscholtzii isolates from skin scrapings, nail clippings, and blood.MethodsThe nine isolates were identified based on colony morphology, light microscopy, and internal transcribed spacer (ITS)-based phylogeny. In vitro antifungal susceptibility of the fungal isolates was evaluated by the Etest to determine the minimum inhibitory concentration (MIC).ResultsThe nine isolates examined were confirmed as D. eschscholtzii. They exhibited typical features of Daldinia sp. on Sabouraud Dextrose Agar, with white felty colonies and black-gray coloration on the reverse side. Septate hyphae, branching conidiophore with conidiogenous cells budding from its terminus, and nodulisporium-like conidiophores were observed under the microscope. Phylogenetic analysis revealed that the nine isolates were clustered within the D. eschscholtzii species complex. All the isolates exhibited low MICs against azole agents (voriconazole, posaconazole, itraconazole, and ketoconazole), as well as amphotericin B, with MIC of less than 1 µg/ml.DiscussionEarly and definitive identification of D. eschscholtzii is vital to reducing misuse of antimicrobial agents. Detailed morphological and molecular characterization as well as antifungal profiling of D. eschscholtzii provide the basis for future studies on its biology, pathogenicity, and medicinal potential.

【 授权许可】

CC BY