Assessment of plasma B7-H3 levels in pediatric patients with different degrees of surgical stress

BMC Pediatrics

Research Article

Jiang Huai Wang¹  Qing Yuan²  Xing Feng³  Jie Huang⁴  Jian Wang⁵  Yan Li⁵  Xue Guang Zhang⁶  Yi Ping Li⁷  Jian Pan⁷ 

[1] Department of Academic Surgery, University College Cork, Cork University Hospital, Cork, Ireland;Department of Anesthesiology, Affiliated Children’s Hospital, Soochow University, 215003, Suzhou, China;Department of Neonatology, Affiliated Children’s Hospital, Soochow University, 215003, Suzhou, China;Department of Pediatric Cardiology, Affiliated Children’s Hospital, Soochow University, 215003, Suzhou, China;Department of Pediatric Surgery, Affiliated Children’s Hospital, Soochow University, 215003, Suzhou, China;Institute of Medical Biotechnology, Soochow University, 215006, Suzhou, China;Institute of Pediatric Research, Affiliated Children’s Hospital, Soochow University, 215003, Suzhou, China;

关键词: Pediatric surgery;    B7-H3;    Surgical stress;    Children;   

DOI  :  10.1186/s12887-016-0655-1

 received in 2015-01-22, accepted in 2016-07-21,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundSurgical stress initiates a series of host hormone, metabolism and immune responses, which predominantly affect the homeostatic mechanism of patients with major surgery. B7-H3 is a co-stimulatory molecule and has been shown to participate in both adaptive and innate immune responses. In this study we evaluated the clinical significance of plasma B7-H3 levels in pediatric patients with different types of operation and degrees of surgical stress.MethodsA total of 48 children received pediatric general and cardiac surgery were recruited into this study. Based on the surgical stress scoring, children were divided into moderate stress (n = 14) and severe stress (n = 34) groups. Plasma B7-H3 levels were assessed at selected time points: before surgery, immediately after surgery, at day 1, day 3, and day 7 after surgery. Correlations between plasma B7-H3 levels and surgical stress scores were also examined.ResultsPlasma B7-H3 levels were significantly decreased in all 48 pediatric patients after surgery compared to the B7-H3 level before surgery (p < 0.01). Children with general surgery showed significant decreases in plasma B7-H3 immediately after surgery, and at day 3 and day 7 after surgery (p < 0.05, p < 0.01), whereas children with cardiac surgery showed reduced plasma B7-H3 immediately after surgery and at day 3 after surgery (p < 0.05). Plasma B7-H3 in cardiac surgery group was dropped much lower than that in general surgery group at day 1 (p < 0.05) and day 3 (p < 0.01) after surgery. Significantly reduced plasma B7-H3 was observed in the severe stress group, but not in the moderate stress group, immediately after surgery and at day 3 after surgery (p < 0.05), and severe stress group had significantly lower plasma B7-H3 levels than moderate stress group at day 1, day 3, and day 7 after surgery (p < 0.05). Furthermore, plasma B7-H3 levels at day 1 (p = 0.01) and day 3 (p = 0.025) after surgery correlated negatively with surgical stress scores.ConclusionsPlasma B7-H3 levels were decreased significantly in children subjected to pediatric general and cardiac surgery, which is closely associated with the severity of surgical stress. The negative correlation of plasma B7-H3 levels at day 1 and day 3 after surgery with surgical stress scoring implicates that the plasma B7-H3 level might be a useful biomarker for monitoring stress intensity during pediatric surgery.

【 授权许可】

CC BY   
© Li et al. 2016