Chemical composition and evaluation of the antibacterial and Cytotoxic activities of the essential oil from the leaves of Myracrodruon urundeuva

BMC Complementary and Alternative Medicine

Research Article

Ítalo Diego Rebouças de Araújo¹  José Veríssimo Fernandes¹  Vânia Sousa Andrade¹  Andreza Conceição Véras de Aguiar Guerra²  Raimundo Fernandes de Araújo Júnior²  Renata Mendonça Araújo³  Renato Ferreira de Almeida Júnior⁴  Nayara Coriolano de Aquino⁵  Kléber Juvenal Silva Farias⁶ 

[1] Centro de Biociências (CB), Departamento de Microbiologia e Parasitologia, Universidade Federal do Rio Grande do Norte (UFRN), 59072-970, Natal, RN, Brazil;Centro de Biociências (CB), Departamento de Morfologia, Universidade Federal do Rio Grande do Norte (UFRN), 59072-970, Natal, RN, Brazil;Centro de Ciências Exatas e da Terra (CCET), Instituto de Química, Universidade Federal do Rio Grande do Norte (UFRN), 59072-970, Natal, RN, Brazil;Centro de Ciências da Saúde (CCS), Departamento de Análises Clínicas e Toxicológicas, Universidade Federal do Rio Grande do Norte (UFRN), 59072-970, Natal, RN, Brazil;Centro de Ciências, Departamento de Química Orgânica e Inorgânica, Universidade Federal do Ceará (UFC), 60021-940, Fortaleza, CE, Brazil;Instituto de Medicina Tropical (IMT), Universidade Federal do Rio Grande do Norte (UFRN), 59072-970, Natal, RN, Brazil;

关键词: Myracrodruon urundeuva;    Bactericidal activity;    Minimum inhibitory concentration;    Chemical characterization;    Essential oil;    Cytotoxicity;   

DOI  :  10.1186/s12906-017-1918-6

 received in 2017-01-18, accepted in 2017-08-10,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundThis study evaluated the in vitro activity of essential oil extracted from the leaves of Myracrodruon urundeuva.MethodsThe oil was obtained by hydro-distillation and characterized by Gas Chromatography coupled to Mass Spectrometry (GC-MS) and Gas Chromatography with Flame Ionization Detector (GC-FID). The antibacterial activity was evaluated by the broth microdilution technique and the MIF was determined by using growth indicator CTT (2,3,5-triphenyl-tetrazolium) and CBM in BHI agar. The oil’s cytotoxicity was evaluated in HeLa, HEK-293, and Vero E6 cells using MTT, 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl tetrazolium.ResultsThe oil showed chemical markers, including α-pinene (87.85%), trans-caryophyllene (1.57%), limonene (1.49%) and β -pinene (1.42%), and activity against all strains: Staphylococcus aureus (MIC = MBC = 0.22 mg/mL), Staphylococcus epidermidis (MIC = 0.11 mg/mL and MBC = 0.22 mg/mL), Escherichia coli (MIC = 0.88 mg/mL and MBC = 1.75 mg/mL), Pseudomonas aeruginosa (MIC = MBC = 7 mg/mL) and Salmonella Enteritidis (MIC = MBC = 0.44 mg/mL). In vitro cytotoxicity tests showed that the oil is not toxic and has slight antitumor activity.ConclusionsWe conclude that the M. urundeuva oil results are promising, with prospects of being pharmacologically viable.

【 授权许可】

CC BY   
© The Author(s). 2017