Simultaneous quantification and antiatherosclerosis effect of the traditional Korean medicine, Hwangryunhaedok-tang

BMC Complementary and Alternative Medicine

Research Article

Hyeun-Kyoo Shin¹  Chang-Seob Seo¹  Ohn Soon Kim¹  Jung-Hoon Kim² 

[1] Herbal Medicine Formulation Research Group, Korea Institute of Oriental Medicine, 1672 Yuseong-daero Yuseong-gu, 305-811, Daejeon, South Korea;Herbal Medicine Formulation Research Group, Korea Institute of Oriental Medicine, 1672 Yuseong-daero Yuseong-gu, 305-811, Daejeon, South Korea;Division of Pharmacology, School of Korean Medicine, Pusan National University, Yangsan, 626-870, Gyeongnam, Republic of Korea;

关键词: Simultaneous quantification;    Hwangryunhaedok-tang;    Antiatherosclerosis effect;    HPLC–PDA;    Traditional Korean medicine;   

DOI  :  10.1186/s12906-015-0632-5

 received in 2014-07-15, accepted in 2015-03-24,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundHwangryunhaedok-tang (HHT) is a traditional herbal medicine that is used for the treatment of fever, inflammation, gastritis, and hypertension. In this study, we performed simultaneous determination of the five components, geniposide (1), baicalin (2), coptisine (3), palmatine (4), and berberine (5) in HHT by using a high-performance liquid chromatography–photodiode array (HPLC–PDA) analysis. We also evaluated the antioxidative activity of HHT and compounds 1–5 by measuring their effects on low-density lipoprotein (LDL) oxidation and antiproliferative abilities in vascular smooth muscle cells (VSMCs).MethodsFive compounds were separated within 40 min by using a Gemini C18 column (temp. 35°C; two-component gradient elution; flow rate 1.0 mL/min; detector 240 and 277 nm). The activities of HHT and compounds 1–5 were tested with the radical scavengers 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt and 2,2-diphenyl-1-picrylhydrazyl, in thiobarbituric acid reactive substance assays, and in relative electrophoretic mobility assays using CuSO4-induced LDL oxidation systems. The antiproliferative effects of samples on platelet-derived growth factor (PDGF)-induced VSMC proliferation were studied by using a cell proliferation assay.ResultsRegression analysis of the five major compounds showed good linearity (r2 ≥ 0.9997) in different concentration ranges. The recoveries of the five compounds were in the range 86.31–110.78%, with relative standard deviations below 2.1%; those of intra- and interday precision were 0.04–3.78% and 0.04–1.69%, respectively. HHT reduced the oxidation properties of LDL induced by CuSO4 and inhibited cell proliferation in PDGF-treated VSMCs. Among the five components, compound 2 could effectively suppress LDL oxidation and PDGF-induced VSMC proliferation.ConclusionsThe established HPLC–PDA method will help to improve quality control of HHT. The results demonstrate that HHT has antiatherosclerotic activity and that it functions by modulating LDL oxidation and VSMC proliferation. The effects of HHT may be attributed, at least I part, to compound 2.

【 授权许可】

Unknown   
© Seo et al.; licensee BioMed Central. 2015. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.