Simultaneous quantification and antiatherosclerosis effect of the traditional Korean medicine, Hwangryunhaedok-tang

BMC Complementary and Alternative Medicine

Hyeun-Kyoo Shin²  Jung-Hoon Kim¹  Ohn Soon Kim²  Chang-Seob Seo² 

[1] Division of Pharmacology, School of Korean Medicine, Pusan National University, Yangsan, Gyeongnam 626-870, Republic of Korea;Herbal Medicine Formulation Research Group, Korea Institute of Oriental Medicine, 1672 Yuseong-daero Yuseong-gu, Daejeon 305-811, South Korea

关键词: Traditional Korean medicine;    HPLC–PDA;    Antiatherosclerosis effect;    Hwangryunhaedok-tang;    Simultaneous quantification;   

Others  :  1172055
DOI  :  10.1186/s12906-015-0632-5

 received in 2014-07-15, accepted in 2015-03-24,  发布年份 2015

【 摘 要 】

Background

Hwangryunhaedok-tang (HHT) is a traditional herbal medicine that is used for the treatment of fever, inflammation, gastritis, and hypertension. In this study, we performed simultaneous determination of the five components, geniposide (1), baicalin (2), coptisine (3), palmatine (4), and berberine (5) in HHT by using a high-performance liquid chromatography–photodiode array (HPLC–PDA) analysis. We also evaluated the antioxidative activity of HHT and compounds 1–5 by measuring their effects on low-density lipoprotein (LDL) oxidation and antiproliferative abilities in vascular smooth muscle cells (VSMCs).

Methods

Five compounds were separated within 40 min by using a Gemini C₁₈ column (temp. 35°C; two-component gradient elution; flow rate 1.0 mL/min; detector 240 and 277 nm). The activities of HHT and compounds 1–5 were tested with the radical scavengers 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt and 2,2-diphenyl-1-picrylhydrazyl, in thiobarbituric acid reactive substance assays, and in relative electrophoretic mobility assays using CuSO₄-induced LDL oxidation systems. The antiproliferative effects of samples on platelet-derived growth factor (PDGF)-induced VSMC proliferation were studied by using a cell proliferation assay.

Results

Regression analysis of the five major compounds showed good linearity (r² ≥ 0.9997) in different concentration ranges. The recoveries of the five compounds were in the range 86.31–110.78%, with relative standard deviations below 2.1%; those of intra- and interday precision were 0.04–3.78% and 0.04–1.69%, respectively. HHT reduced the oxidation properties of LDL induced by CuSO₄ and inhibited cell proliferation in PDGF-treated VSMCs. Among the five components, compound 2 could effectively suppress LDL oxidation and PDGF-induced VSMC proliferation.

Conclusions

The established HPLC–PDA method will help to improve quality control of HHT. The results demonstrate that HHT has antiatherosclerotic activity and that it functions by modulating LDL oxidation and VSMC proliferation. The effects of HHT may be attributed, at least I part, to compound 2.

【 授权许可】

   
2015 Seo et al.; licensee BioMed Central.

【 图 表 】

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