Isolation, identification and characterization of Paenibacillus polymyxa CR1 with potentials for biopesticide, biofertilization, biomass degradation and biofuel production

BMC Microbiology

Research Article

Jacqueline MacDonald¹  Brian Weselowski²  Naeem Nathoo³  Ze-Chun Yuan⁴  Alexander William Eastman⁴ 

[1] Department of Microbiology & Immunology, Dental Science Building Rm. 3014, University of Western Ontario, N6A 5C1, London, ON, Canada;London Research and Development Centre, Agriculture & Agri-Food Canada, 1391 Sandford Street, N5V 4T3, London, ON, Canada;London Research and Development Centre, Agriculture & Agri-Food Canada, 1391 Sandford Street, N5V 4T3, London, ON, Canada;Department of Biology, Biological and Geological Sciences Building, University of Western Ontario, N6A 5B7, London, ON, Canada;London Research and Development Centre, Agriculture & Agri-Food Canada, 1391 Sandford Street, N5V 4T3, London, ON, Canada;Department of Microbiology & Immunology, Dental Science Building Rm. 3014, University of Western Ontario, N6A 5C1, London, ON, Canada;

关键词: Paenibacillus polymyxa;    plant growth promotion;    PGPR;    antagonism;    biocontrol;    biopesticide;    biological nitrogen fixation;    diazotroph;    biofertilizer;    biomass degradation;   

DOI  :  10.1186/s12866-016-0860-y

 received in 2016-06-22, accepted in 2016-10-07,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundPaenibacillus polymyxa is a plant-growth promoting rhizobacterium that could be exploited as an environmentally friendlier alternative to chemical fertilizers and pesticides. Various strains have been isolated that can benefit agriculture through antimicrobial activity, nitrogen fixation, phosphate solubilization, plant hormone production, or lignocellulose degradation. However, no single strain has yet been identified in which all of these advantageous traits have been confirmed.ResultsP. polymyxa CR1 was isolated from degrading corn roots from southern Ontario, Canada. It was shown to possess in vitro antagonistic activities against the common plant pathogens Phytophthora sojae P6497 (oomycete), Rhizoctonia solani 1809 (basidiomycete fungus), Cylindrocarpon destructans 2062 (ascomycete fungus), Pseudomonas syringae DC3000 (bacterium), and Xanthomonas campestris 93-1 (bacterium), as well as Bacillus cereus (bacterium), an agent of food-borne illness. P. polymyxa CR1 enhanced growth of maize, potato, cucumber, Arabidopsis, and tomato plants; utilized atmospheric nitrogen and insoluble phosphorus; produced the phytohormone indole-3-acetic acid (IAA); and degraded and utilized the major components of lignocellulose (lignin, cellulose, and hemicellulose).ConclusionsP. polymyxa CR1 has multiple beneficial traits that are relevant to sustainable agriculture and the bio-economy. This strain could be developed for field application in order to control pathogens, promote plant growth, and degrade crop residues after harvest.

【 授权许可】

CC BY   
© The Author(s). 2016