Interactional mechanisms of Paenibacillus polymyxa SC2 and pepper (Capsicum annuum L.) suggested by transcriptomics

BMC Microbiology

Binghai Du¹  Hu Liu¹  Kai Liu¹  Yufei Li¹  Chengqiang Wang¹  Yanqin Ding¹  Ke Ge¹ 

[1] College of Life Sciences, Shandong Engineering Research Center of Plant-Microbia Restoration for Saline-Alkali Land, Shandong Key Laboratory of Agricultural Microbiology, National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, Shandong Agricultural University, 271018, Tai’an, China;

关键词: Paenibacillus polymyxa;    Capsicum annuum;    PGPR;    Interaction mechanisms;    RNA-seq;   

DOI  :  10.1186/s12866-021-02132-2

来源: Springer

【 摘 要 】

BackgroundPaenibacillus polymyxa SC2, a bacterium isolated from the rhizosphere soil of pepper (Capsicum annuum L.), promotes growth and biocontrol of pepper. However, the mechanisms of interaction between P. polymyxa SC2 and pepper have not yet been elucidated. This study aimed to investigate the interactional relationship of P. polymyxa SC2 and pepper using transcriptomics.ResultsP. polymyxa SC2 promotes growth of pepper stems and leaves in pot experiments in the greenhouse. Under interaction conditions, peppers stimulate the expression of genes related to quorum sensing, chemotaxis, and biofilm formation in P. polymyxa SC2. Peppers induced the expression of polymyxin and fusaricidin biosynthesis genes in P. polymyxa SC2, and these genes were up-regulated 2.93- to 6.13-fold and 2.77- to 7.88-fold, respectively. Under the stimulation of medium which has been used to culture pepper, the bacteriostatic diameter of P. polymyxa SC2 against Xanthomonas citri increased significantly. Concurrently, under the stimulation of P. polymyxa SC2, expression of transcription factor genes WRKY2 and WRKY40 in pepper was up-regulated 1.17-fold and 3.5-fold, respectively.ConclusionsThrough the interaction with pepper, the ability of P. polymyxa SC2 to inhibit pathogens was enhanced. P. polymyxa SC2 also induces systemic resistance in pepper by stimulating expression of corresponding transcription regulators. Furthermore, pepper has effects on chemotaxis and biofilm formation of P. polymyxa SC2. This study provides a basis for studying interactional mechanisms of P. polymyxa SC2 and pepper.

【 授权许可】

CC BY