Biophysiochemical properties of endothelial cells cultured on bio-inspired collagen films

BMC Biotechnology

Methodology Article

Eunseop Yeom¹  Seungchul Lee¹  Kyung Won Seo¹  Jung-Eun Gil²  Young-Ran Ha³  Eunseok Seo³  Sang Joon Lee⁴ 

[1] Center for Biofluid and Biomimic Research, Department of Mechanical Engineering, Pohang University of Science and Technology, San 31, Hyoja-dong, Nam-Gu, 790-784, Pohang, Gyeongbuk, South Korea;Department of Biological Sciences, Center for Cognition and Sociality, Institute for Basic Science, 291 Daehak-ro, 305-701, Yuseong-gu, Daejeon, South Korea;Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, San 31, Hyoja-dong, Nam-Gu, 790-784, Pohang, Gyeongbuk, Korea;Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, San 31, Hyoja-dong, Nam-Gu, 790-784, Pohang, Gyeongbuk, Korea;Center for Biofluid and Biomimic Research, Department of Mechanical Engineering, Pohang University of Science and Technology, San 31, Hyoja-dong, Nam-Gu, 790-784, Pohang, Gyeongbuk, South Korea;

关键词: Root Mean Square;    Enhance Green Fluorescent Protein;    Flow Chamber;    Collagen Film;    Endothelial Cell Surface;   

DOI  :  10.1186/1472-6750-14-61

 received in 2014-01-29, accepted in 2014-06-26,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundIn this study, we investigated the effect of the extracellular matrix on endothelial dysfunction by careful observation of human umbilical vein endothelial cells (HUVECs) cultured on denatured collagen film.ResultsHUVECs on denatured collagen film showed relatively high surface roughness compared with normal HUVECs. The expression levels of MMP-1, MMP-2 and CD146 increased in the ECs on denatured collagen film. In addition, we examined the accumulation of fluorescent beads on HUVEC layers subjected to circulatory flow. The number of accumulated fluorescent beads increased on the disorganized HUVEC layers.ConclusionsThe proposed in vitro study using bio-inspired collagen films could potentially be used in the size- and ligand-based design of drugs to treat endothelial dysfunction caused by circulatory vascular diseases.

【 授权许可】

Unknown   
© Seo et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.