Genome-wide transcriptional analysis of T cell activation reveals differential gene expression associated with psoriasis

BMC Genomics

Research Article

Eduardo Fonseca¹  Carlos Ferrándiz²  Emilia Fernández³  Lluís Puig⁴  Nuria Palau⁵  Raül Tortosa⁵  Antonio Julià⁵  Sara Marsal⁵  María López-Lasanta⁵ 

[1] Dermatology Service, Hospital Abente y Lago, 15001, La Coruña, Spain;Dermatology Service, Hospital Universitari Germans Trias i Pujol, 08916, Badalona, Spain;Dermatology Service, Hospital Universitario de Salamanca, 37007, Salamanca, Spain;Dermatology Service, Hospital de la Santa Creu i Sant Pau, 08040, Barcelona, Spain;Rheumatology Research Group, Vall d’Hebron Research Institute, 08035, Barcelona, Spain;

关键词: Psoriasis;    T cell;    Gene expression;    Microarray;    Genetic pathway;    Gene network;   

DOI  :  10.1186/1471-2164-14-825

 received in 2013-06-17, accepted in 2013-11-19,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundPsoriasis is a chronic autoimmune disease in which T cells have a predominant role in initiating and perpetuating the chronic inflammation in skin. However, the mechanisms that regulate T cell activation in psoriasis are still incompletely understood. The objective of the present study was to characterize the main genetic pathways associated with T cell activation in psoriasis.ResultsGene expression profiles from in vitro activated T cells were obtained from 17 psoriasis patients and 7 healthy controls using Illumina HT-12 v4 microarrays. From a total of 47,321 analyzed transcripts, 42 genes were found to be differentially expressed between psoriasis and controls (FDR p-value < 0.1, absolute fold-change > 1.2). Using an independent cohort of 8 patients and 8 healthy controls we validated the overexpression of SPATS2L (p-value =0.0009) and KLF6 (p-value =0.0012) genes in activated T cells from psoriasis patients. Using weighted correlation analysis we identified SPATS2L and KLF6 coexpression networks, which were also significantly associated with psoriasis (p-value < 0.05). Gene Ontology analysis allowed the identification of several biological processes associated with each coexpression network. Finally, using Gene Set Enrichment Analysis over the global T cell transcriptome we also found additional genetic pathways strongly associated with psoriasis (p-value < 0.0001).ConclusionsThis study has identified two new genes, SPATS2L and KLF6, strongly associated with T cell activation in psoriasis. Functional analyses of the gene expression profiles also revealed new biological processes and genetic pathways associated with psoriasis. The results of this study provide an important insight into the biology of this common chronic inflammatory disease.

【 授权许可】

Unknown   
© Palau et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.